Differentially Expressed lncRNAs After the Activation of Primordial Follicles in Mouse
Differentially Expressed lncRNAs After the Activation of Primordial Follicles in Mouse
复制标题
小鼠原始卵泡激活后差异表达的lncRNA
DOI：
10.1177/1933719118805869
复制
发表时间：
2019
影响因子：
2.9
通讯作者：
Zhang Xiaoning
中科院分区：
文献类型：
--
作者：
Zheng Liping;Luo Ruichen;Su Tie;Hu Liaoliao;Gao Fengxin;Zhang Xiaoning
原始卵泡的激活对卵巢卵泡发育至关重要，直接影响女性的生育能力和生殖寿命。一些研究表明长链非编码rna （lncRNAs）在卵巢功能中的作用。然而，lncrna在原始卵泡起始中的确切参与尚不清楚。本研究采用体外培养模型来研究lncrna在原始卵泡激活中的作用。我们发现，体外培养8天后，小鼠卵巢第3天的原始卵泡被激活，卵巢形态发生变化，原始卵泡数量增加，哺乳动物不育20样激酶信使RNA （mRNA）下调，生长分化因子9 mRNA上调。接下来，我们通过转录组水平的RNA测序检测了lncRNA的表达谱，发现在体外培养8天的3天卵巢中，与第3天小鼠的卵巢相比，60078个lncRNA中，6541个lncRNA上调，2135个lncRNA下调。我们还发现，在培养的卵巢中，4171个mrna上调，1795个mrna下调。基因本体和通路分析表明，差异表达lncRNA靶点和mrna的功能与卵巢发育相关的许多过程和通路密切相关，包括细胞增殖分化、发育过程以及其他信号转导通路。此外，许多新鉴定的lncRNAs显示可诱导表达，这表明这些lncRNAs可能是研究小鼠原始卵泡激活的良好候选者。本研究为进一步探索lncrna在小鼠原始卵泡启动中的相关机制提供了基础。
The activation of primordial follicles is critical to ovarian follicle development, which directly influences female fertility and reproductive life span. Several studies have suggested a role for long noncoding RNAs (lncRNAs) in ovarian function. However, the precise involvement of lncRNAs in the initiation of primordial follicles is still unknown. Here, an in vitro culture model was used to investigate the roles of lncRNAs in primordial follicle activation. We found that primordial follicles in day 3 mouse ovaries were activated after culturing for 8 days in vitro, as indicated by ovarian morphology changes, increases in primary follicle number, and downregulation of mammalian Sterile 20-like kinase messenger RNA (mRNA) and upregulation of growth differentiation factor 9 mRNA. We next examined lncRNA expression profiles by RNA sequencing at the transcriptome level and found that among 60 078 lncRNAs, 6541 lncRNA were upregulated and 2135 lncRNA were downregulated in 3-day ovaries cultured for 8 days in vitro compared with ovaries from day 3 mice. We also found that 4171 mRNAs were upregulated and 1795 were downregulated in the cultured ovaries. Gene ontology and pathway analyses showed that the functions of differentially expressed lncRNA targets and mRNAs were closely linked with many processes and pathways related to ovary development, including cell proliferation and differentiation, developmental processes, and other signaling transduction pathways. Additionally, many novel identified lncRNAs showed inducible expression, suggesting that these lncRNAs may be good candidates for investigating mouse primordial follicle activation. This study provides a foundation for further exploring lncRNA-related mechanisms in the initiation of mouse primordial follicles.