In vitro evaluation of the sinus sagittalis superior thrombosis model in the rat using 3D micro- and nanocomputed tomography
In vitro evaluation of the sinus sagittalis superior thrombosis model in the rat using 3D micro- and nanocomputed tomography
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DOI：
10.1007/s00234-009-0617-5
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发表时间：
2010-09-01
期刊：
影响因子：
2.8
通讯作者：
Gerriets, Tibo
中科院分区：
文献类型：
--
作者：
Langheinrich, Alexander Claus;Yeniguen, Mesut;Gerriets, Tibo
脑静脉和脑窦血栓形成是中风的常见原因。动物模型帮助我们了解这种情况的潜在病理生理学。因此，我们研究的目的是利用微纳米计算机断层扫描（CT）成像来评估一个成熟的矢状窦（SSS）血栓形成模型。4只大鼠进行SSS血栓形成实验。造影剂灌注后，分离大脑，使用（8 μ m）A(3)体素尺寸的微型ct扫描，生成脑血管系统的3D图像。为了更详细地了解血管灌注区域，我们使用纳米ct成像来研究对比增强血管和闭塞静脉的边界层。获得SSS血栓组的静脉和动脉血管体积分数和灰度测量，并与对照组进行比较。用方差分析检验血管体积分数和灰度测量值差异的显著性。结果与组织学相辅相成。显微ct被证明可以准确地显示和区分SSS血栓模型中的血管闭塞区域。此外，三维显微ct提供了动脉和静脉血管体积分数的定量信息。显微ct成像能够在SSS血栓模型中实现并发症（心室破裂）的全三维可视化。我们建立了灰度测量方法，通过该方法可以对局灶性脑缺血进行影像学分类（p < 0.001）。利用纳米ct，可以可视化对比灌注静脉和闭塞静脉的界面。显微ct对局灶性脑缺血动物模型灌注区域的分析和区分是可行的。
Thrombosis of the cerebral veins and sinus are common causes of stroke. Animal models help us to understand the underlying pathophysiology of this condition. Therefore, the purpose of our study was to evaluate a well-established model for sinus sagittalis (SSS) thrombosis using micro- and nanocomputed tomography (CT) imaging.SSS thrombosis was performed in four rats. After contrast perfusion, brains were isolated and scanned using micro-CT at (8 A mu m)A(3) voxel size to generate 3D images of the cerebral vasculature. For more detailed information on vascular perfusion territories, nano-CT imaging was performed to investigate the boundary layer of contrast-enhanced vessels and the occluded veins. The venous and arterial vascular volume fraction and gray scale measurements were obtained in the SSS thrombosis group and compared to controls. The significance of differences in vascular volume fraction and gray scale measurements was tested with analysis of variance. Results were complemented with histology.Micro-CT proved to accurately visualize and differentiate vascular occlusion territories performed in the SSS thrombosis model. Moreover, 3D micro-CT provided quantitative information on arterial and venous vascular volume fraction. Micro-CT imaging enables a total 3D visualization of complications (ventricle rupture) in the SSS thrombosis model. We established gray scale measurements by which focal cerebral ischemia could be radiographically categorized (p < 0.001).Using nano-CT, the interface of contrast-perfused and occluded veins can be visualized. Micro-CT is feasible for analysis and differentiation of perfusion territories in an animal model of focal cerebral ischemia.