Effects of MRI Contrast Agents on the Stem Cell Phenotype
Effects of MRI Contrast Agents on the Stem Cell Phenotype
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DOI：
10.3727/096368910x494623
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Himmelreich, Uwe
中科院分区：
文献类型：
--
作者：
Crabbe, Annelies;Vandeputte, Caroline;Himmelreich, Uwe
缺血性中风的最终治疗是恢复缺血区域的血液供应和再生丢失的神经细胞。这可以通过移植分化成血管或神经元细胞类型的细胞，或分泌增强内源性干细胞/祖细胞的自我更新、募集、长期存活和功能整合的营养因子来实现。已经开发了实验性中风模型来确定基于干/祖细胞的疗法的潜在有益效果。为了跟踪移植细胞在体内的命运，已经开发了许多非侵入性成像方法。磁共振成像（MRI）是一种高分辨率、临床相关的方法，可以在体内监测用造影剂标记的细胞。在这项研究中，比较了三种不同的（超）小超顺磁性氧化铁[（U）SPIO]颗粒（Resovist（R）、Endorem（R）、Sinerem（R））对三种不同干细胞群[小鼠胚胎干细胞（mESC）、大鼠多能成体祖细胞（rMAPC）和小鼠间充质干细胞（mMSC）]的标记效率。用Resovist（R）和Endorem（R）的标记效率在不同干细胞之间显著不同。当用基于MRI的可视化所需的颗粒浓度标记时，用允许通过体内MRI检测细胞的范围内的（U）SPIO标记不影响干细胞的分化。最后，我们证明了标记的rMAPC可以在体内检测到，标记并不干扰它们的迁移。我们得出结论，成功使用（U）SPIO进行基于MRI的可视化将需要评估每个个体（干）细胞群体的最佳（U）SPIO，以确保最灵敏的检测，而不会产生相关毒性。
The ultimate therapy for ischemic stroke is restoration of blood supply in the ischemic region and regeneration of lost neural cells. This might be achieved by transplanting cells that differentiate into vascular or neuronal cell types, or secrete trophic factors that enhance self-renewal, recruitment, long-term survival, and functional integration of endogenous stem/progenitor cells. Experimental stroke models have been developed to determine potential beneficial effect of stem/progenitor cell-based therapies. To follow the fate of grafted cells in vivo, a number of noninvasive imaging approaches have been developed. Magnetic resonance imaging (MRI) is a high-resolution, clinically relevant method allowing in vivo monitoring of cells labeled with contrast agents. In this study, labeling efficiency of three different stem cell populations [mouse embryonic stem cells (mESC), rat multipotent adult progenitor cells (rMAPC), and mouse mesenchymal stem cells (mMSC)] with three different (ultra)small superparamagnetic iron oxide [(U)SPIO] particles (Resovist (R), Endorem (R), Sinerem (R)) was compared. Labeling efficiency with Resovist (R) and Endorem (R) differed significantly between the different stem cells. Labeling with (U)SPIOs in the range that allows detection of cells by in vivo MRI did not affect differentiation of stem cells when labeled with concentrations of particles needed for MRI-based visualization. Finally, we demonstrated that labeled rMAPC could be detected in vivo and that labeling did not interfere with their migration. We conclude that successful use of (U)SPIOs for MRI-based visualization will require assessment of the optimal (U)SPIO for each individual (stem) cell population to ensure the most sensitive detection without associated toxicity.