Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome
Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome
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DOI：
10.2144/03356st02
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发表时间：
2003-12-01
期刊：
影响因子：
2.7
通讯作者：
Davisson, MT
中科院分区：
文献类型：
--
作者：
Liu, DP;Schmidt, C;Davisson, MT
Ts65Dn小鼠是唐氏综合征的节段性三体模型。到目前为止，Ts65Dn小鼠已经通过培养的外周淋巴细胞的染色体分析或荧光原位杂交（FISH）的费力方法鉴定。我们在这里报告的定量PCR方法的Ts65Dn小鼠基因分型，以及表型描述，视觉预分类小鼠基因分型。
The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description,for visually preclassifying mice to be genotyped.