Plasma angiotensin II levels at moment of drinking during angiotensin II intravenous infusion.
Plasma angiotensin II levels at moment of drinking during angiotensin II intravenous infusion.
复制标题
血管紧张素 II 静脉输注期间饮酒时的血浆血管紧张素 II 水平。
DOI：
10.1152/ajpregu.1988.255.3.r500
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Phillips,MI
中科院分区：
文献类型：
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作者：
Anke,J;VanEekelen,M;Phillips,MI
血管紧张素Ⅱ（ANG Ⅱ）在10-200 ng·kg ~（-1）·min ~（-1）的表观生理剂量范围内静脉注射诱导大鼠饮水反应。为了测定饮酒时的血浆ANG II水平，在饮酒反应开始时从股动脉收集血液样品。输注前以类似方式获得对照血样。最低剂量的ANG II没有引起饮酒。在相对较长的饮水潜伏期（40-65 min）后，观察到25、50和75 ng ANG II.kg-1. min-1引起的可变饮水量。当输注100 ng ANG Ⅱ.kg ~（-1）. min ~（-1）或更高剂量的ANG Ⅱ时，所有动物均显示饮水反应。饮酒时间与ANG II剂量呈负相关，200 ng ANG II.kg-1. min-1时短至8 min。饮酒大鼠血浆中ANG II的测量结果显示，所有浓度均相似，平均值为458 +/- 58.1 pg/ml。该致渴血浆ANG II水平相当于脱水48小时后的血浆ANG II水平。结果表明，响应于外源性静脉血管紧张素II的饮酒需要达到阈值水平，该阈值水平相当于当脱水延长时内源性肾素-血管紧张素系统产生的水平。这表明，静脉内血管紧张素II不参与瞬间流体稳态，但只有在脱水严重时才起作用。
Angiotensin II (ANG II) was infused intravenously within an apparent physiological dose range of 10-200 ng.kg-1.min-1 to induce a drinking response in rats. To determine the plasma ANG II level at the moment of drinking, blood samples were collected from the femoral artery at the onset of the drinking response. Control blood samples were obtained in a similar way before infusion. The lowest dose of ANG II did not evoke drinking. Variable water intake in response to 25, 50, and 75 ng ANG II.kg-1.min-1 after a relatively long latency to drink (40-65 min) was observed. All animals showed a drinking response when 100 ng ANG II.kg-1.min-1 or higher doses of ANG II were infused. The latencies to drink were inversely correlated to the dose of ANG II and were as short as 8 min with 200 ng ANG II.kg-1.min-1. Measurement of ANG II in the plasma of drinking rats showed that all concentrations were similar with an average of 458 +/- 58.1 pg/ml. This dipsogenic plasma ANG II level is equivalent to plasma ANG II after 48 h of dehydration. The results show that drinking in response to exogenous intravenous ANG II requires a threshold level to be reached that is equivalent to levels produced by the endogenous renin-angiotensin system when dehydration is prolonged. This suggests that intravenous ANG II is not involved in moment-to-moment fluid homeostasis but operates only when dehydration is severe.