Echinococcosis in pigs and intestinal infection with Echinococcus spp. in dogs in southwestern Lithuania
Echinococcosis in pigs and intestinal infection with Echinococcus spp. in dogs in southwestern Lithuania
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DOI：
10.1016/j.vetpar.2008.11.011
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发表时间：
2009-03-23
影响因子：
2.6
通讯作者：
Deplazes, P.
中科院分区：
文献类型：
--
作者：
Bruzinskaite, R.;Sarkunas, M.;Deplazes, P.
囊状棘球蚴病是许多东欧和亚洲国家的一种主要新发人畜共患病。对立陶宛684头猪肝的屠宰后检查显示，家庭农场动物细粒棘球绦虫感染率（13.2%; 95%CI 10.7-16.2）显著高于工业化农场动物（4.1%; 95%CI 0.8-11.5）。患病率也显着高于1岁以上的猪比年轻的。此外，在0.5%的家庭农场的猪中，不育和钙化E。PCR鉴定多房性病变。用两种方法对12个村177个家庭农场的240只家犬粪便中带绦虫卵进行了调查。与改良的McMaster方法（n = 12）相比，采用漂浮/筛分方法（n = 34）诊断出排泄带绦虫卵的犬显著更多。用从34只狗的粪便中分离的带绦虫卵的DNA进行多重PCR，发现26例带绦虫感染，9、E.细粒棘球绦虫2例，多房棘球绦虫2例（合并带绦虫4例）。和E.细粒棘球绦虫或多房棘球绦虫感染）。E. 7头猪、1头牛和E. 8只狗粪便中的颗粒虫卵在所有情况下均显示基因型G6/7（“猪/骆驼品系”）。棘球绦虫的高感染压力。在家庭农场中，需要启动控制程序。(C)2008 Elsevier B. V.保留所有权利。
Cystic echinococcosis is a major emerging zoonosis in many Eastern European and Asian countries. Post slaughter examinations of 684 pig livers in Lithuania revealed significantly higher numbers of Echinococcus granulosus infections in animals from family farms (13.2%; 95% CI 10.7-16.2) as compared with those from industrial farms (4.1%; 95% CI 0.8-11.5). The prevalence was also significantly higher in pigs older than I year than in younger ones. In addition, in 0.5% of the pigs from the family farms, infertile and calcified E. multilocularis lesions were identified by PCR. Faecal samples from rural dogs (n = 240) originating from 177 family farms in 12 villages were investigated for taeniid eggs with two methods. Significantly more dogs excreting taeniid eggs were diagnosed with the flotation/sieving method (n = 34) as compared to the modified McMaster method (n = 12). Multiplex PCR performed with DNA from taeniid eggs isolated from faeces of 34 dogs revealed 26 infections with Taenia spp., 9 with E. granulosus and 2 with E multilocularis (4 cases with concurrent Taenia spp. and E. granulosus or E multilocularis infections). Genotyping of E. granulosus cyst tissues from 7 pigs, I head of cattle and from E. granulosus eggs from 8 dog faeces revealed the genotype G6/7 ('pig/camel strain') in all cases. The high infection pressure with Echinococcus spp. in family farms necessitates initiating control programs. (C) 2008 Elsevier B.V. All rights reserved.