Freshly isolated rat alveolar type I cells, type II cells, and cultured type II cells have distinct molecular phenotypes
Freshly isolated rat alveolar type I cells, type II cells, and cultured type II cells have distinct molecular phenotypes
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DOI：
10.1152/ajplung.00272.2004
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Dobbs, L
中科院分区：
文献类型：
--
作者：
Gonzalez, R;Yang, YH;Dobbs, L
我们使用Affymetrix大鼠芯片的微阵列分析来确定新鲜分离的大鼠I型（Ti）和TII细胞以及培养的TII细胞的基因表达谱。我们的目标是1）描述Ti和TiI细胞的分子表型“指纹”，2），以深入了解两种细胞类型通过差异表达的基因之间可能的功能差异，3）识别可能表明Ti细胞潜在功能的基因，由于对这种细胞类型知之甚少，而4）确定培养的TII细胞和新鲜分离的Ti细胞之间基因表达的相似性和差异。对于这些实验，我们使用了包含的分离的Ti和Tii细胞的制剂
We used microarray analysis with Affymetrix rat chips to determine gene expression profiles of freshly isolated rat type I (TI) and TII cells and cultured TII cells. Our goals were 1) to describe molecular phenotypic "fingerprints" of TI and TII cells, 2) to gain insight into possible functional differences between the two cell types through differentially expressed genes, 3) to identify genes that might indicate potential functions of TI cells, since so little is known about this cell type, and 4) to ascertain the similarities and differences in gene expression between cultured TII cells and freshly isolated TI cells. For these experiments, we used preparations of isolated TI and TII cells that contained