Increased expression of interleukin-8 mRNA in ulcerative colitis and Crohn's disease mucosa and epithelial cells.
Increased expression of interleukin-8 mRNA in ulcerative colitis and Crohn's disease mucosa and epithelial cells.
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DOI：
10.1002/ibd.3780010106
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发表时间：
1995-01-01
影响因子：
4.9
通讯作者：
Macdermott, R P
中科院分区：
文献类型：
--
作者：
Izutani, R;Loh, E Y;Macdermott, R P
：白介素 8 (IL-8) 是一种趋化细胞因子（趋化因子），可吸引并激活粒细胞。 IL-8 由于其对失活的相对抵抗力和体内半衰期长，可能在炎症性肠病 (IBD) 的发生和持续中发挥核心作用。使用定量逆转录酶聚合酶链反应 (RT-PCR) 测定，我们观察到从溃疡性结肠炎 (UC) 和克罗恩病 (CD) 患者粘膜炎症活跃的手术切除标本中获得的结肠粘膜切片中 IL-8 mRNA 水平升高。 UC 和 CD 患者肠粘膜活检中 IL-8 mRNA 的表达水平在受累的粘膜切片中比非受累的粘膜切片高得多。 RT-PCR 检测到的 IL-8 mRNA 表达最高的是 UC 粘膜和 UC 患者分离的肠上皮细胞。 IBD 肠粘膜细胞和 IBD 上皮细胞产生的 IL-8 增加可能与 UC 和 CD 患者发炎肠道中粒细胞的持续吸引和激活有关。 IL-8 等趋化因子是有效的趋化分子，可能在 IBD 炎症的增强和持续中发挥核心作用。
: Interleukin-8 (IL-8) is a chemotactic cytokine (chemokine), which both attracts and activates granulocytes. IL-8 could have a central function in the initiation and perpetuation of the inflammatory bowel diseases (IBD), due to its relative resistance to inactivation and long half-life in vivo. Using a quantitative reverse transcriptase polymerase chain reaction (RT-PCR) assay, we have observed elevated levels of IL-8 mRNA in colonic mucosal sections obtained from surgically resected specimens from ulcerative colitis (UC) and Crohn's disease (CD) patients with actively inflamed mucosa. The level of IL-8 mRNA expression in the intestinal mucosal biopsies from UC and CD patients was much greater in involved as opposed to noninvolved mucosal sections. The highest expression of IL-8 mRNA detected by RT-PCR was in UC mucosa and in isolated intestinal epithelial cells from UC patients. Increased IL-8 production by cells in IBD intestinal mucosa as well as IBD epithelial cells may be involved in the continuous attraction and activation of granulocytes in the inflamed intestine in both UC and CD patients. Chemokines, such as IL-8, are potent chemoattractant molecules and may have a central role in the augmentation and perpetuation of inflammation in IBD.