Isolation and characterization of pos mutants defective in correct positioning of septum in Schizosaccharomyces pombe
Isolation and characterization of pos mutants defective in correct positioning of septum in Schizosaccharomyces pombe
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DOI：
10.2108/zsj.13.235
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发表时间：
1996-04-01
影响因子：
0.9
通讯作者：
Toyoshima, YY
中科院分区：
文献类型：
--
作者：
Edamatsu, M;Toyoshima, YY
我们已经分离出了分裂酵母的突变体，这些突变体在隔膜的正确定位方面有缺陷。在目测筛选中，我们获得了16个在限制性温度下表现出不等分隔的克隆，它们被分为三个互补群。在限制性温度下，所有突变体都能正常进行核分裂。然而，在胞质分裂中，在不依赖于有丝分裂纺锤体的位点形成收缩环。这些结果表明，胞质分裂的位置信息没有准确地传递到细胞赤道。此外，所有突变体都经常表现出不正确的方向和/或变形的隔，这表明隔的位置是密切相关的正确的方向和组织的隔。
We have isolated mutants of fission yeast defective in correct positioning of septum. In visual screening, we obtained 16 clones showing unequal septation at restrictive temperature, which were classified into three complementation groups. At restrictive temperature, all the mutants underwent nuclear division normally. In cytokinesis, however, a contractile ring was formed at the site independent of the mitotic spindle. These results suggest that positional information for cytokinesis are not accurately transmitted to the cell equator. Furthermore, all the mutants frequently displayed incorrect orientation and/or distortion of septum, which suggests that the septum positioning is closely related to correct orientation and organization of septum.