Megakaryocyte Quantitation
Megakaryocyte Quantitation
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巨核细胞定量
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通讯作者：
Lauren Harr
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作者：
Lauren Harr
描述了一种用于巨核细胞定量的方法。在该技术中，对先前注射的血浆结合59 Fe的最大骨髓吸收时获得的骨髓切片的放射性以及巨核细胞和有核红细胞的数量进行计数。我们通过将巨核细胞：放射性比率与采样时的总骨髓59 Fe活性相关联来计算总巨核细胞。对切片材料中的细胞数进行定量时，需要一个与细胞直径和切片厚度相关的多重计数校正因子。大鼠的正常巨核细胞数为11.0 × 106/kg，人为6.1 × 106/kg。
A method is described for the quantitation of megakaryocytes. In this technic bone marrow sections obtained at the time of maximal marrow uptake of previously injected plasma bound 59Fe are counted for radioactivity as well as the number of megakaryocytes and nucleated red cells. We calculated the total megakaryocytes by relating the ratio of megakaryocyte: radioactivity with the total marrow 59Fe activity at the time of sampling. A multiple counting correction factor which is related to cell diameter and section thickness is required for quantitation of cell num-bers from section material. The normal megakaryocyte number for the rat is 11.0 x 106/kg, and 6.1 x 106/kg for man.