cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.
cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.
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具有潜在跨膜片段的新型人基质金属蛋白酶的 cDNA 序列和 mRNA 组织分布。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
B. Hinzmann
中科院分区：
文献类型：
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作者：
H. Will;B. Hinzmann
从人肺cDNA文库中分离到一种新型基质金属蛋白酶的互补DNA序列。它由3530 bp组成，编码669个氨基酸的多肽。与其他基质金属蛋白酶相比，推断出的氨基酸链序列与最近发现的具有582个氨基酸的膜型基质金属蛋白酶最为相似。同样，它由一个信号肽、一个原结构域、一个催化结构域、一个血红素同源结构域和一个c端结构域组成。此外，新型基质金属蛋白酶与其582个氨基酸同源物具有相似的激活位点，在催化区域插入8个氨基酸，在c端附近有20多个疏水氨基酸。c端结构域的疏水结构表明这种新型基质金属蛋白酶也是膜结合的。当用针对669个氨基酸链重组片段的多克隆抗体在免疫印迹中探测肺细胞膜组分时，M(r) 72000蛋白优先与抗体反应。Northern-blot分析表明，两种膜型基质金属蛋白酶mRNA在组织中的分布有很大差异。582氨基酸的mRNA主要在肺、胎盘、肾脏、卵巢、肠、前列腺和脾脏中合成，而669氨基酸的mRNA则主要在肝脏、胎盘、睾丸、结肠和肠道中合成。在胰腺、肾脏、肺、心脏和骨骼肌中也检测到大量的后一种mRNA。
The complementary DNA sequence of a novel matrix metalloproteinase was isolated from a human lung cDNA library. It consists of 3530 bp and encodes a polypeptide of 669 amino acids. In comparison to other matrix metalloproteinases, the deduced sequence of the amino acid chain exhibits closest similarity to a recently discovered membrane-type matrix metalloproteinase of 582 amino acids. Likewise, it is composed of a signal peptide, a prodomain, a catalytic domain, a hemopexin-homologous domain and a C-terminal domain. Furthermore, the novel matrix metalloproteinase shares a similar activation site with its 582-amino-acid homologue, an insertion of eight amino acids in the catalytic domain and a tract of more than 20 hydrophobic amino acids near the C-terminus. The hydrophobic structure in the C-terminal domain suggests that the novel matrix metalloproteinase is also membrane bound. When lung cell membrane fractions were probed in immunoblots with polyclonal antibodies against a recombinant fragment of the 669-amino-acid chain, a protein of M(r) 72,000 reacted preferentially with the antibodies. Northern-blot analysis demonstrated quite different tissue distributions of mRNA for the two membrane-type matrix metalloproteinases. While mRNA for the 582-amino-acid enzyme was found predominantly in lung, placenta, kidney, ovary, intestine, prostate and spleen, mRNA for the 669-amino-acid enzyme appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts of the latter mRNA were also detected in pancreas, kidney, lung, heart and skeletal muscle.
DOI：
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发表时间：
1993-11
期刊：
The Journal of biological chemistry
影响因子：
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作者：
S. Shapiro;D. Kobayashi;T. Ley
通讯作者：
S. Shapiro;D. Kobayashi;T. Ley
DOI：
10.1016/s0021-9258(18)47324-3
发表时间：
1994-10
期刊：
The Journal of biological chemistry
影响因子：
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作者：
D. Pei;G. Majmudar;S. Weiss
通讯作者：
D. Pei;G. Majmudar;S. Weiss