Human decidual stromal cells secrete C-X-C motif chemokine 13, express B cell-activating factor and rescue B lymphocytes from apoptosis: distinctive characteristics of follicular dendritic cells
Human decidual stromal cells secrete C-X-C motif chemokine 13, express B cell-activating factor and rescue B lymphocytes from apoptosis: distinctive characteristics of follicular dendritic cells
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DOI：
10.1093/humrep/des198
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发表时间：
2012-09-01
影响因子：
6.1
通讯作者：
Olivares, E. -G.
中科院分区：
文献类型：
--
作者：
Munoz-Fernandez, R.;Prados, A.;Olivares, E. -G.
蜕膜基质细胞（DSC）通常被认为是成纤维细胞，尽管它们的功能、细胞谱系和起源尚不完全清楚。我们之前证明，人类 DSC 与滤泡树突状细胞 (FDC) 表现出相似之处：DSC 表达 FDC 相关抗原，两种类型的细胞都具有收缩性，并且都与间充质干细胞 (MSC) 相关。为了进一步表征 DSC，我们研究了 DSC 和 FDC 是否具有任何独特的表型和功能特征。人 FDC 系从扁桃体切除样本中获得，人 DSC 系从选择性终止妊娠样本中获得，人 MSC 系从骨髓抽吸物中获得。我们分离了 DSC、FDC 和 MSC 系，并通过流式细胞术和酶联免疫吸附测定比较了它们的特征。细胞系与肿瘤坏死因子 (TNF) 和淋巴毒素 (LT)(12)（参与 FDC 分化的细胞因子）一起培养。暴露于黄体酮和 cAMP（参与 DSC 分化（蜕膜化）的因子）后，细胞系也在培养物中分化。与 MSC 一样，DSC 和 FDC 也表达 MSC 相关抗原（CD10、CD29、CD54、CD73、CD106、β-平滑肌肌动蛋白和 STRO-1），但缺乏 CD45 表达，所有三种类型的细胞系均表现出 CD54 (ICAM-1) 表达增加和 TNF 和 LT12 培养时的 CD106 (VCAM-1)。然而，DSC 和 FDC 表现出 MSC 中未观察到的特征：DSC 表达 FDC 相关抗原 CD14、CD21 和 CD23、B 细胞激活因子并分泌 C-X-C 基序趋化因子 13。此外，DSC 系而非 MSC 系抑制 B 淋巴细胞的自发凋亡，这是 FDC 的典型功能属性。在用黄体酮和 cAMP 培养期间，FDC 与 DSC 类似，但与 MSC 不同，其形态从成纤维细胞变为圆形，并且细胞分泌催乳素。我们的结果表明 DSC 和 FDC 在 MSC 中具有共同的前体，但该前体在归巢于外周组织时获得新的能力。我们在怀孕期间免疫内分泌调节的背景下讨论这些共同的特性。
Decidual stromal cells (DSCs) have classically been considered fibroblastic cells, although their function, cell lineage and origin are not fully understood. We previously demonstrated that human DSCs showed similarities with follicular dendritic cells (FDCs): DSCs expressed FDC-associated antigens, both types of cells are contractile and both are related to mesenchymal stem cells (MSCs). To further characterize DSCs, we investigated whether DSCs and FDCs share any distinctive phenotypical and functional characteristics.Human FDC lines were obtained from tonsillectomy samples, human DSC lines from elective termination of pregnancy samples and human MSC lines from bone marrow aspirates. We isolated DSC, FDC and MSC lines and compared their characteristics with flow cytometry and enzyme-linked immunosorbent assay. Cell lines were cultured with tumour necrosis factor (TNF) and lymphotoxin (LT)(12), cytokines involved in FDC differentiation. Cell lines were also differentiated in culture after exposure to progesterone and cAMP, factors involved in the differentiation (decidualization) of DSC.Like MSCs, DSCs and FDCs expressed MSC-associated antigens (CD10, CD29, CD54, CD73, CD106, -smooth muscle actin and STRO-1) and lacked CD45 expression, and all three types of cell line showed increased expression of CD54 (ICAM-1) and CD106 (VCAM-1) when cultured TNF and LT12. DSCs and FDCs, however, exhibited characteristics not observed in MSCs: DSCs expressed FDC-associated antigens CD14, CD21 and CD23, B cell-activating factor and secreted C-X-C motif chemokine 13. Moreover, DSC lines but not MSC lines inhibited the spontaneous apoptosis of B lymphocytes, a typical functional attribute of FDC. During culture with progesterone and cAMP, FDCs, like DSCs but in contrast to MSCs, changed their morphology from a fibroblastic to a rounder shape, and cells secreted prolactin.Our results suggest that DSCs and FDCs share a common precursor in MSCs but this precursor acquires new capacities when it homes to peripheral tissues. We discuss these shared properties in the context of immuneendocrine regulation during pregnancy.