Neonicotinoid-induced pathogen susceptibility is mitigated by Lactobacillus plantarum immune stimulation in a Drosophila melanogaster model.
Neonicotinoid-induced pathogen susceptibility is mitigated by Lactobacillus plantarum immune stimulation in a Drosophila melanogaster model.
复制标题
DOI：
10.1038/s41598-017-02806-w
复制
发表时间：
2017-06-02
影响因子：
4.6
通讯作者：
Reid G
中科院分区：
文献类型：
--
作者：
Daisley BA;Trinder M;McDowell TW;Welle H;Dube JS;Ali SN;Leong HS;Sumarah MW;Reid G
农药广泛用于粮食生产，以最大限度地提高农作物产量。然而，新烟碱类杀虫剂会对蜜蜂（Apis mellifera）产生无意的毒性，这可能部分与被称为蜂群崩溃综合症的种群数量大幅下降有关。我们假设，吡虫啉（常见的新烟碱类杀虫剂；IMI）暴露会使果蝇（蜜蜂的昆虫模型）更容易受到细菌病原体、热应激和肠道菌群失调的影响。我们的结果表明，免疫缺陷（Imd）途径对于黑腹果蝇响应 IMI 毒性的生存是必需的。 IMI 暴露引起宿主微生物群的改变，如本土醋杆菌和乳杆菌的增加所指出的。此外，当同时暴露于细菌感染和热应激（37°C）时，亚致死暴露于 IMI 会导致黑腹果蝇存活率下降。与对照相比，这与 TotA 和 Dpt（分别是 Imd 下游促生存基因和抗菌基因）表达的加剧增加相一致。向暴露于 IMI 的黑腹果蝇补充植物乳杆菌 ATCC 14917 可减轻粘质沙雷氏菌（细菌病原体）脓毒症感染后的生存缺陷。这些发现支持新烟碱类杀虫剂的潜在毒性以及益生菌乳酸菌降低 IMI 诱导的感染易感性的潜力。
Pesticides are used extensively in food production to maximize crop yields. However, neonicotinoid insecticides exert unintentional toxicity to honey bees (Apis mellifera) that may partially be associated with massive population declines referred to as colony collapse disorder. We hypothesized that imidacloprid (common neonicotinoid; IMI) exposure would make Drosophila melanogaster (an insect model for the honey bee) more susceptible to bacterial pathogens, heat stress, and intestinal dysbiosis. Our results suggested that the immune deficiency (Imd) pathway is necessary for D. melanogaster survival in response to IMI toxicity. IMI exposure induced alterations in the host-microbiota as noted by increased indigenous Acetobacter and Lactobacillus spp. Furthermore, sub-lethal exposure to IMI resulted in decreased D. melanogaster survival when simultaneously exposed to bacterial infection and heat stress (37 °C). This coincided with exacerbated increases in TotA and Dpt (Imd downstream pro-survival and antimicrobial genes, respectively) expression compared to controls. Supplementation of IMI-exposed D. melanogaster with Lactobacillus plantarum ATCC 14917 mitigated survival deficits following Serratia marcescens (bacterial pathogen) septic infection. These findings support the insidious toxicity of neonicotinoid pesticides and potential for probiotic lactobacilli to reduce IMI-induced susceptibility to infection.