Estrogen receptor beta (ERbeta) is expressed differently from ERalpha in bovine placentomes.
Estrogen receptor beta (ERbeta) is expressed differently from ERalpha in bovine placentomes.
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雌激素受体β (ERbeta) 在牛胎盘中的表达与 ERα 不同。
DOI：
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发表时间：
2005
影响因子：
1.8
通讯作者：
B. Hoffmann
中科院分区：
文献类型：
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作者：
G. Schuler;U. Teichmann;A. Taubert;K. Failing;B. Hoffmann
牛胎盘雌激素的功能仍然知之甚少。为了研究通过雌激素受体 β (ERbeta) 作为胎盘生长、分化和功能的局部调节剂的推定作用，在从妊娠中期（第 110-150 天；n=7）、妊娠晚期（第 180-280 天；n=9）的奶牛获得的牛胎盘组中测定了这种 ER 亚型的表达，这些胎盘组取自预产奶牛（在母体预产期下降后立即取样）。血液中黄体酮水平变得明显；n=3)，而正常足月奶牛 (n=5) 的蛋白质和 mRNA 水平也变得明显。通过使用抗人ERbeta单克隆抗体（克隆PPG5/10）的免疫组织化学方法，在胎盘的胎儿（子叶）和母体（肉阜）成分的多种细胞类型中发现了核信号，其中在成熟滋养层巨细胞（TGC）、胎儿和母体血管细胞以及肉阜基质细胞中强度最高，其中阳性细胞的百分比从58.9+/-2.3增加到58.9+/-2.3。预产期和产期奶牛在妊娠中期和晚期为 59.1+/-3.3 至 65.1+/-4.9 和 69.4+/-4.2。 （p<0.0001）。仅在预产期和产期动物中观察到单核滋养层细胞的清晰染色。常规 RT-PCR 证实了所研究的所有阶段的肉阜和子叶中的 ERbeta 表达。通过半定量实时 RT-PCR，与肉阜相比，在子叶中测量到更高水平的 ERbeta 特异性 mRNA（p<0.01）。结果显示，与仅在肉阜中表达的ERα相反，ERβ也在子叶中广泛表达，这表明ERβ主要在TGC分化和胎盘血管生成和/或血管功能中发挥作用。
The functions of placental estrogens in cattle are still poorly understood. In order to investigate a putative role as local regulators of placental growth, differentiation and functions via estrogen receptor beta (ERbeta), the expression of this ER isoform was determined in bovine placentomes obtained from cows at midgestation (days 110-150; n=7), late gestation (days 180-280; n=9), from prepartal cows (sampling immediately after the prepartal decline in maternal progesterone blood levels became obvious; n=3) and from cows at normal term (n=5) on the protein and mRNA level. By means of immunohistochemistry using a monoclonal antibody against human ERbeta (clone PPG5/10), nuclear signals were found in numerous cell types of the fetal (cotyledon) and maternal (caruncle) component of the placentomes with highest intensities in mature trophoblast giant cells (TGC), fetal and maternal vascular cells and caruncular stromal cells, in which the percentage of positive cells increased from 58.9+/-2.3 and 59.1+/-3.3 at mid- and late gestation to 65.1+/-4.9 and 69.4+/-4.2 in prepartal and parturient cows, resp. (p<0.0001). Clear staining of uninucleated trophoblast cells was only observed in prepartal and parturient animals. Conventional RT-PCR confirmed ERbeta expression in caruncles and cotyledons at all phases under investigation. With semi-quantitative real-time RT-PCR higher levels of ERbeta-specific mRNA were measured in cotyledons compared to caruncles (p<0.01). The results show that in contrast to ERalpha, which is only expressed in the caruncle, ERbeta is also extensively expressed in the cotyledon and suggest a role of ERbeta primarily in TGC differentiation and placental angiogenesis and/or vascular functions.