Impact of oocyte cryopreservation on embryo development
Impact of oocyte cryopreservation on embryo development
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DOI：
10.1016/j.fertnstert.2009.01.148
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发表时间：
2010-01-15
影响因子：
6.7
通讯作者：
Gianaroli, Luca
中科院分区：
文献类型：
--
作者：
Magli, M. Cristina;Lappi, Michela;Gianaroli, Luca
目的：验证卵母细胞解冻后受精卵和胚胎的形态学评价能否为其发育性能提供相关信息。设计：比较来自兄弟姐妹新鲜卵母细胞和冷冻卵母细胞的受精、受精卵和胚胎形态。地点：意大利博洛尼亚意大利医学会生育医学研究中心生殖医学处。患者：234例患者接受了卵浆内单精子注射周期，其中1101个中期备用卵母细胞被冷冻保存。随后进行256次循环解冻，共解冻997个卵母细胞。干预措施：对新鲜和冷冻卵母细胞进行卵浆内单精子注射。主要观察指标：受精率、原核受精卵形态、胚胎卵裂率。结果：无论女性年龄大小，解冻卵母细胞与兄弟姐妹新鲜卵母细胞相比，受精卵发育成高质量受精卵和定期分裂胚胎的机会较低。结果，冷冻循环中转移周期的百分比明显低于新鲜循环（分别为79%和93%）；移植优质胚的比例也有相同的趋势。结论：与兄弟姐妹的新鲜卵母细胞相比，冷冻周期中受精率和卵裂率的降低表明，即使在解冻后存活下来，缓慢冷冻的过程也会对进一步生长的潜力产生负面影响，这在第一次卵裂分裂时就很明显了。[j] .中国农业科学（英文版），2010;33(3):591 - 596。(C)2010年，美国生殖医学学会。)
Objective: To verify whether the morphologic evaluation of zygotes and embryos derived from thawed oocytes could provide some relevant information regarding their developmental performance.Design: Fertilization, zygote, and embryo morphology from sibling fresh and frozen oocytes was compared.Setting: Reproductive Medicine Unit, Societa Italiana Studi Medicina della Riproduzione, Bologna, Italy.Patient(s): Two hundred thirty-four patients underwent intracytoplasmic sperm injection cycles from which 1,101 spare metaphase II oocytes were cryopreserved. Subsequently, 256 thawing cycles were per-formed, and 997 oocytes were thawed.Intervention(s): Intracytoplasmic sperm injection was performed on both fresh and frozen oocytes.Main Outcome Measure(s): Fertilization rates, pronuclear zygote morphology, and embryo cleavage rates.Result(s): Thawed oocytes had lower chances of being fertilized and developing into top-quality zygotes and regularly cleaving embryos when compared with sibling fresh oocytes irrespective of female age. As a result, the percentage of transferred cycles was significantly lower in frozen cycles compared with fresh cycles (79% and 93%, respectively); the proportion of transferred top-quality embryos followed the same trend.Conclusion(s): Reduced fertilization and cleavage rates in frozen cycles when compared with sibling fresh oocytes suggest that, even if surviving thawing, the process of slow freezing has a negative impact on the potential of further growth that is evident as early as the first cleavage divisions. (Fertil Steril(R) 20 10;93:510-6. (C)2010 by American Society for Reproductive Medicine.)