Abundance and distribution of ammonia-oxidizing microorganisms in the sediments of Beiyun River, China
Abundance and distribution of ammonia-oxidizing microorganisms in the sediments of Beiyun River, China
复制标题
北运河沉积物中氨氧化微生物的丰度及分布
DOI：
10.1007/s13213-016-1191-9
复制
发表时间：
2016-09-01
影响因子：
3
通讯作者：
Chen, Yongjuan
中科院分区：
文献类型：
--
作者：
Bao, Linlin;Wang, Xiaoyan;Chen, Yongjuan
氨氧化由氨氧化古菌（AOA）和氨氧化细菌（AOB）驱动，在全球氮循环中发挥着重要作用。然而，氨氧化微生物的人口和分布在城市河流沉积物中的密集人为氮输入仍然不清楚。本研究比较了北运河夏季和冬季沉积物中AOA和AOB的多样性和丰度。AOB在数量上占绝对优势，两种基因的丰度冬季均高于夏季，AOA群落的物种多样性高于AOB。系统发育分析表明，亚硝化螺菌属（Nitrosospirasp.）夏季优势AOB为亚硝化单胞菌（Nitrosomonasp.）冬季优势种，但未发现AOA的特有属。杨洼水闸底泥中的主要污染物和微生物群落结构与其他站点不同。克隆文库显示，相当一部分amoA序列与污水处理厂系统中的序列相匹配。硝酸盐含量的季节变化与氨氮丰度的季节变化呈显著正相关（P< 0.05）。总氮、总有机碳、铵态氮和pH是影响北运河沉积物中氨氧化微生物群落组成差异的主要环境因子。这些研究结果表明，污水输入，水闸运行，和季节变化可能是决定的AOB和AOA在城市河流沉积物中的丰度和分布的关键因素。
Ammonia oxidation, driven by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), plays an important role in the global nitrogen cycle. However, the population and distribution of ammonia-oxidizing microorganisms in the sediments of urban rivers with intensive anthropogenic nitrogen inputs is still unclear. In this study, we compared the diversity and abundance of AOA and AOB in Beiyun River sediments from summer to winter. AOB dominated numerically over AOA and the abundance of both theamoAgenes were much higher in winter than in summer, while AOA communities were more phylogenetically diverse than AOB in this study area. Phylogenetic analysis revealed thatNitrosospirasp. was the dominant AOB in summer, withNitrosomonassp. dominant in winter, while no specific genus was found for AOA in present study. The main pollutants and microbial communities in the sediments deriving from Yangwa Watergate were different from other sites. Clone libraries showed that a considerable percentage ofamoAsequences matched closely with those from wastewater treatment plant (WWTP) systems. The seasonal change in nitrate content was significantly and positively related to the seasonal variation inamoAabundance (P< 0.05). Total nitrogen, total organic carbon, ammonium, and pH were the main environmental factors accounting for the difference in community composition of ammonia-oxidizing microorganisms in the sediments of Beiyun River. These findings suggest that effluent inputs, water gate operation, and seasonal changes might be key factors determining the abundance and distribution of AOB and AOA in the sediments of urban rivers.