Thermal effects of transcranial near-infrared laser irradiation on rabbit cortex
Thermal effects of transcranial near-infrared laser irradiation on rabbit cortex
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DOI：
10.1016/j.neulet.2013.07.049
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发表时间：
2013-10-11
影响因子：
2.5
通讯作者：
Zivin, Justin A.
中科院分区：
文献类型：
--
作者：
Chen, Yongmei;De Taboada, Luis;Zivin, Justin A.
经颅近红外激光治疗（TLT）可改善动物模型中的卒中结局。适当的激光剂量是发挥治疗效果所必需的。然而，应用更高的激光能量可能会导致皮质组织加热并加剧中风损伤。本研究旨在探讨经颅近红外激光治疗的热效应和安全性。用波长为808 nm的半导体激光器向家兔大脑皮层发射不同功率密度的激光。在2 min经颅激光照射期间，使用热探头监测和测量皮质温度。激光治疗后24 h，组织学染色观察神经病理改变。经颅连续波（CW）激光以22.2和55.6 mW/cm（2）的功率密度照射家兔大脑皮层2 min，未引起皮层温度升高。在相同的治疗方案下，111.1 mW/cm（2）的皮层功率密度在2分钟的照射时间内使脑温逐渐升高0.5 ℃，并在照射后1-2分钟内恢复到基线值。另外，在22.2 mW/cm（2）CW和111.1 mW/cm（2）脉冲波（PW）的三重激光照射后评价组织学染色，显示正常的神经细胞形态。本研究证明，目前用于动物卒中研究的TLT功率不会导致皮质组织发热和组织病理学损伤。(C)2013作者由Elsevier爱尔兰有限公司出版。保留所有权利。
Transcranial near-infrared laser therapy (TLT) improves stroke outcome in animal models. Adequate laser doses are necessary to exert therapeutic effects. However, applying higher laser energy may cause cortical tissue heating and exacerbate stroke injury. The objective of this study is to examine the thermal effect and safety of transcranial near-infrared laser therapy. Diode laser with a wavelength of 808 nm was used to deliver different power densities to the brain cortex of rabbits. Cortical temperature was monitored and measured using a thermal probe during the 2 min transcranial laser irradiation. Neuro-pathological changes were examined with histological staining 24 h after laser treatment. Transcranial laser irradiation for 2 min at cortical power densities of 22.2 and 55.6 mW/cm(2) with continuous wave (CW) did not increase cortical temperature in rabbits. With the same treatment regime, cortical power density at 111.1 mW/cm(2) increased brain temperature gradually by 0.5 degrees C over the 2 min exposure and returned to baseline values within 1-2 min post-irradiation. Separately, histological staining was evaluated after triple laser exposure of 22.2 mW/cm(2) CW and 111.1 mW/cm(2) pulse wave (PW) and showed normal neural cell morphology. The present study demonstrated that the TLT powers currently utilized in animal stroke studies do not cause cortical tissue heating and histopathological damage. (C) 2013 The Authors. Published by Elsevier Ireland Ltd. All rights reserved.