Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells
Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells
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DOI：
10.1016/s0891-5849(02)01082-1
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发表时间：
2002-11-15
影响因子：
7.4
通讯作者：
Hjelmeland, LM
中科院分区：
文献类型：
--
作者：
Weigel, AL;Handa, JT;Hjelmeland, LM
氧化应激在眼后极衰老疾病中起关键作用，如老年性黄斑变性。已针对少数基因研究了体外培养的RPE细胞的氧化应激反应。然而，全面的转录反应还没有被阐明。这项研究的目的是确定ARPE-19细胞暴露于三种主要的氧化应激物质过氧化氢(H_2O_2)、4-羟基壬烯醛(HNE)和叔丁基氢过氧化氢(TBH)是否会改变一组常见基因的转录。正如预期的那样，观察到了一个共同的反应，包括由所有三个处理差异调节的35个基因。在这些基因中，只有一个基因上调，而且只被一种氧化剂上调，而所有其他反应都是下调。这些基因主要分为五大类：细胞凋亡、细胞周期调控、细胞间通讯、信号转导和转录调控。此外，大量基因仅受一种氧化剂的差异调节，包括Clontech Human 1.2微阵列上存在的大多数常规氧化应激反应基因。这项研究对涉及使用单一氧化剂和单一细胞培养条件的结果的一般性提出了质疑。(C)2002年爱思唯尔科学公司。
Oxidative stress plays a key role in aging diseases of the posterior pole of the eye such as age-related macular degeneration. The oxidative stress response of in vitro RPE cells has been studied for a small number of genes. However, a comprehensive transcriptional response has yet to be elucidated. The purpose of this study was to determine if the transcription of a common set of genes is altered by exposure of ARPE- 19 cells to three major generators of oxidative stress, hydrogen peroxide (H2O2), 4-hydroxynonenal (HNE), and tert-butylhydroperoxide (tBH). As expected, a common response was observed that included 35 genes differentially regulated by all three treatments. Of these, only one gene was upregulated, and only by one oxidant, while all other responses were downregulation. The majority of these genes fell into five functional categories: apoptosis, cell cycle regulation, cell-cell communication, signal transduction, and transcriptional regulation. Additionally, a large number of genes were differentially regulated by one oxidant only, including the majority of the conventional oxidative stress response genes present on the Clontech Human 1.2 microarray. This study raises questions regarding the generality of results that involve the use of a single oxidant and a single cell culture condition. (C) 2002 Elsevier Science Inc.