Molecular definition in a somatic cell hybrid of a specific 2:13 translocation breakpoint in childhood rhabdomyosarcoma.
Molecular definition in a somatic cell hybrid of a specific 2:13 translocation breakpoint in childhood rhabdomyosarcoma.
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儿童横纹肌肉瘤中特定 2:13 易位断点的体细胞杂交体的分子定义。
作者：
C. Mitchell;J. A. Ventris;T. Warr;J. K. Cowell
在肺泡横纹肌肉瘤中发现了一致、平衡、相互的染色体易位 t(2:13) (q35:q14)。已在携带 (2:13) 易位的横纹肌肉瘤细胞系和小鼠 3T3 细胞之间构建了体细胞杂交体。一种杂交细胞系显示保留了衍生 (13:2) 染色体，但分离了正常 13 号染色体和衍生 (2:13) 染色体。使用人类 13 号染色体上可用的 DNA 探针，我们发现视网膜母细胞瘤、酯酶 D、p7D2、pG24E6.8 和 pG14E1.9 的位点位于 13q14 断点的远端，而 p7F12、PHU10 和 pG2E3.1 的位点均位于近端。因此，我们定义了大约 28mB 的 13q14 区域，其中包含与此重排相关的断点。
A consistent, balanced, reciprocal chromosomal translocation t(2:13) (q35:q14) has been identified in alveolar rhabdomyosarcoma. Somatic cell hybrids have been constructed between rhabdomyosarcoma cell lines carrying the (2:13) translocation and mouse 3T3 cells. One hybrid cell line was shown to have retained the derivative (13:2) chromosome, but segregated the normal chromosome 13 and the derivative (2:13) chromosome. Using available DNA probes from human chromosome 13 we find that the loci for retinoblastoma, esterase D, p7D2, pG24E6.8 and pG14E1.9 lie distally to the 13q14 breakpoint, whereas those for p7F12, pHU10 and pG2E3.1 all lie proximally. Thus we have defined a region of 13q14 of approximately 28mB which contains the breakpoint associated with this rearrangement.