Detection of apoptotic cells in human colorectal cancer by two different in situ methods: antibody against single-stranded DNA and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) methods.
Detection of apoptotic cells in human colorectal cancer by two different in situ methods: antibody against single-stranded DNA and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) methods.
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DOI：
10.1111/j.1349-7006.1999.tb00732.x
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发表时间：
1999-03
期刊：
影响因子：
--
通讯作者：
Tanigawa N
中科院分区：
文献类型：
--
作者：
Watanabe I;Toyoda M;Okuda J;Tenjo T;Tanaka K;Yamamoto T;Kawasaki H;Sugiyama T;Kawarada Y;Tanigawa N
我们通过两种方法，即传统的末端脱氧核苷酸转移酶（TdT）介导的脱氧尿苷三磷酸（dUTP）-生物素缺口末端标记（TUNEL）法和单链（ss）DNA免疫组织化学法，比较研究了人类结直肠癌中凋亡细胞丢失的程度。TUNEL法测得的凋亡指数（AI）高于ssDNA免疫组化法测得的AI。然而，这两种方法所测得的AI值有显著相关性，晚期癌症中两种方法所测得的AI值均显著高于早期癌症。以增殖细胞核抗原（PCNA）表达的阳性率（PR）评价细胞增殖活性。晚期肿瘤的PR明显高于早期肿瘤。目前的研究结果表明，ssDNA的免疫组化是有用的（是TUNEL法）在结直肠癌中的肿瘤细胞凋亡的评价。此外，证实了在结肠癌从疾病的早期到晚期的进展过程中，不仅增殖活性显著增加，而且肿瘤细胞凋亡也显著增加。
Wecomparatively investigated the extent of apoptotic cell loss in human colorectal cancers evaluated by two methods, namely the conventional terminal deoxynucleotidyl transferase (TdT)‐ mediated deoxyuridine triphosphate (dUTP)‐biotin nick end‐labeling (TUNEL) method and immunohistochemistry for single‐stranded (ss) DNA. The apoptotic index (AI) obtained with the TUNEL method was higher than that shown by the immunohistochemistry for ssDNA. However, a significant correlation in AIs evaluated by these methods was found. The AIs obtained by both methods were significantly higher in the advanced cancers than in the early cancers. Cellular proliferation activity was assessed in terms of positivity rate (PR) for expression of proliferating cell nuclear antigen (PCNA). The PR of advanced cancers was significantly higher than that of early cancers. The present results indicate that immunohistochemistry for ssDNA is useful (as is the TUNEL method) for evaluation of apoptotic tumor cells in colorectal carcinomas. In addition, it was confirmed that there is a remarkable increase of not only proliferation activity, but also tumor cell apoptosis in the process of progression of colon cancer from early to advanced stages of the disease.
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DOI：
10.1016/0921-8777(90)90040-c
发表时间：
1990-07-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
FORTINI, P;BIGNAMI, M;DOGLIOTTI, E
通讯作者：
DOGLIOTTI, E
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
影响因子：
6.4
作者：
FRANKFURT, OS;SECKINGER, D;SUGARBAKER, EV
通讯作者：
SUGARBAKER, EV
影响因子：
7.3
作者：
ANSARI, B;COATES, PJ;HALL, PA
通讯作者：
HALL, PA
影响因子：
--
作者：
Hawkins, N;Lees, J;Ward, R
通讯作者：
Ward, R