NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
复制标题
DOI：
10.1038/s41467-020-18051-1
复制
发表时间：
2020-08-21
影响因子：
16.6
通讯作者：
Zhang, Fan
中科院分区：
文献类型：
--
作者：
Lu, Lingfei;Li, Benhao;Zhang, Fan
生物发光成像在生命科学和生物医学领域有着广泛的应用。然而，传统的生物发光成像通常工作在可见光区，这阻碍了高性能的在体光学成像由于强组织吸收和散射。为了解决这一问题，我们采用生物发光共振能量转移（BRET）和两步荧光共振能量转移（FRET）技术，设计了一种新型的菁染料FD-1029，制备了具有1029 nm第二近红外（NIR-II）区发射的生物发光探针（BP）。生物相容性NIR-II-BP成功应用于小鼠血管和淋巴管成像，其信噪比比NIR-II荧光成像和传统生物发光成像高出约5倍，空间分辨率高出约1.5倍。它们的多重成像能力也得到了很好的显示。利用ATP响应特性，NIR-II-BP能够识别肿瘤转移，肿瘤与正常组织的比率高达83.4。传统的生物发光成像通常工作在可见光区，其性能受到强组织吸收和散射的限制。在这里，作者提出了在第二近红外（NIR-II）区域发射的生物发光探针（BP），并表明NIR-II-BP可以灵敏地识别具有高肿瘤与正常组织比率的肿瘤转移。
Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence imaging usually operates in the visible region, which hampers the high-performance in vivo optical imaging due to the strong tissue absorption and scattering. To address this challenge, here we present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region at 1029nm by employing bioluminescence resonance energy transfer (BRET) and two-step fluorescence resonance energy transfer (FRET) with a specially designed cyanine dye FD-1029. The biocompatible NIR-II-BPs are successfully applied to vessels and lymphatics imaging in mice, which gives similar to 5 times higher signal-to-noise ratios and similar to 1.5 times higher spatial resolution than those obtained by NIR-II fluorescence imaging and conventional bioluminescence imaging. Their capability of multiplexed imaging is also well displayed. Taking advantage of the ATP-responding character, the NIR-II-BPs are able to recognize tumor metastasis with a high tumor-to-normal tissue ratio at 83.4. Conventional bioluminescence imaging usually operates in the visible region and its performance is limited by strong tissue absorption and scattering. Here, the authors present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region, and show the NIR-II-BPs could sensitively recognize tumor metastasis with a high tumor-to-normal tissue ratio.