Nonsense codons within the Rous sarcoma virus gag gene decrease the stability of unspliced viral RNA.
Nonsense codons within the Rous sarcoma virus gag gene decrease the stability of unspliced viral RNA.
复制标题
劳斯肉瘤病毒 gag 基因中的无义密码子会降低未剪接病毒 RNA 的稳定性。
DOI：
10.1128/mcb.11.5.2760-2768.1991
复制
发表时间：
1991
影响因子：
5.3
通讯作者：
Beemon,K
中科院分区：
文献类型：
--
作者：
Barker,GF;Beemon,K
The intracellular accumulation of the unspliced RNA of Rous sarcoma virus was decreased when translation was prematurely terminated by the introduction of nonsense codons within its 5′ proximal gene, thegaggene. In contrast, the levels of spliced viral RNAs were not affected in our transient expression assays in chicken cells. Experiments using the transcription inhibitor dactinomycin showed that mutant unspliced RNAs were degraded more rapidly than wild-type RNA. Furthermore, mutant RNAs could be partially stabilized by coexpression of wild-typegagproteins intrans;however, intactgagproteins were not required to maintain the stability of RNAs which did not contain premature termination codons. Thus, termination codons seemed to destabilize the RNA not because of their effect ongagprotein function but instead because they disrupted the process of translating thegagregion of the RNA. Analysis of double-mutant constructs containing both deletions and termination codons within thegaggene also suggested that the stability of the unspliced RNA was affected by acis-acting interaction between the RNA and ribosomes.
