Proton pathways in green fluorescence protein
Proton pathways in green fluorescence protein
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DOI：
10.1529/biophysj.104.055541
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发表时间：
2005-04-01
影响因子：
3.4
通讯作者：
Agmon, N
中科院分区：
文献类型：
--
作者：
Agmon, N
绿色荧光蛋白（GFP）中的质子途径比以前报道的要扩展。在野生型GFP的X射线数据中，由活性位点到蛋白质表面的两步出口途径，由苏氨酸开关控制。质子进入途径始于GLU-5周围的谷氨酸赖氨酸簇，并一直延伸至活动位点附近的埋入的GLU-222。该结构证据表明，GFP可以用作便携式光驱动的质子泵，质子通过可切换出口途径以激发态发射，并从GLU-222和基态的GLU-5进入途径中进行补充。
Proton pathways in green fluorescent protein (GFP) are more extended than previously reported. In the x-ray data of wild-type GFP, a two-step exit pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton entry pathway begins at a glutamate-lysine cluster around Glu-5, and extends all the way to the buried Glu-222 near the active site. This structural evidence suggests that GFP may function as a portable light-driven proton-pump, with proton emitted in the excited state through the switchable exit pathway, and replenished from Glu-222 and the Glu-5 entry pathway in the ground state.