Isolation, identification, and synthesis of sex pheromone components of female tea cluster caterpillar, Andraca bipunctata Walker (Lepidoptera: Bombycidae) in Taiwan
Isolation, identification, and synthesis of sex pheromone components of female tea cluster caterpillar, Andraca bipunctata Walker (Lepidoptera: Bombycidae) in Taiwan
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DOI：
10.1007/bf02055098
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发表时间：
1996-02-01
影响因子：
2.3
通讯作者：
Chow, YS
中科院分区：
文献类型：
--
作者：
Ho, HY;Tao, YT;Chow, YS
十八醛（18：从台湾茶尺蠖（Andraca bipunctata）的信息素腺体中分离得到（E）-11-十八烯醛（E11-18：Aid）、（E）-14-十八烯醛（E14 - 18：Ald）和（E，E）-11，14-十八碳二烯醛（E11，E14-18：Ald），并通过分析腺体成分及其DMDS加合物的质谱，鉴定为主要成分。各组分的GC保留时间和质谱与真实合成化合物的保留时间和质谱一致。每个雌性腺体（1至3日龄）的18：Ald、E11-18：Ald、E14 - 18：Ald和E11、E14-18：Ald的平均含量分别为121 +/- 76、50 +/- 20、187 +/- 75和237 +/- 110 ng，比例为20：8：31：41。合成Ell，E14-18：在保持诱捕试验中，Ald比其他三种组分或空白对照捕获更多的雄性。E11、E14-18：首次报道Ald为昆虫性信息素。EAG分析表明，雄虫触角对E11、E14-18：Ald反应强烈。此外，在2日龄去头雌蛾体内注射PBAN（信息素生物合成激活神经肽）后4 h，腺体提取液中E11，E14-C18 Aid的含量从0%增加到75.5%，也显著高于未结扎和未注射对照的55.1%。以上结果表明，E11、E14-18：Ald是台湾双点红蜘蛛的性信息素。
Octadecanal (18: Aid), (E)-11-octadecenal (E11-18: Aid), (E)-14-octadecenal (E14-18:Ald) and (E,E)-11,14-octadecadienal (E11,E14-18:Ald) were isolated and identified as major components from the pheromone glands of the tea cluster caterpillar, Andraca bipunctata, in Taiwan by analyzing the mass spectra of gland components and their DMDS adducts. GC retention times and mass spectra of the components were in agreement with those of authentic synthetic compounds. The average amount of 18:Ald, E11-18:Ald, E14-18:Ald and E11,E14-18:Ald per female gland (1 to 3 days old) was 121 +/- 76, 50 +/- 20, 187 +/- 75, and 237 +/- 110 ng, respectively, in a ratio of 20:8:31:41. Synthetic Ell, E14-18:Ald caught more males than each of the other three components or blank control in held trapping tests. E11,E14-18:Ald is reported as an insect sex pheromone for the first time. Male antenna responded to E11, E14-18:Ald strongly in an EAG analysis. Furthermore, 4 hr after the injection of PBAN (pheromone biosynthetic activating neuropeptide) into decapitated female moths (2 days old), the percentage of the E11,E14-C18 Aid in the gland extract increased from 0% to 75.5%, which was also significantly more than that of unligated and uninjected control at 55.1%. All these data indicated that E11,E14-18:Ald is the sex pheromone of the Andraca bipunctata in Taiwan.