Ca 2 +-Dependent Membrane-Binding Proteins in Normal Erythrocytes and Erythrocytes From Patients With Chronic Myelogenous Leukemia

Ca 2 +-Dependent Membrane-Binding Proteins in Normal Erythrocytes and Erythrocytes From Patients With Chronic Myelogenous Leukemia
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正常红细胞和慢性粒细胞白血病患者红细胞中 Ca 2 依赖性膜结合蛋白

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2003
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人红细胞的胞浆和膜组分用抗血清探测,抗血清是针对Caz+依赖性磷脂/膜结合蛋白的膜联蛋白家族的几个成员提出的。抗血清之一,对67 Kd的钙调素,确定红细胞多肽的分子量为48和67 Kd,这是发现在细胞质中正常的红细胞在EGTA的存在下裂解时,但在膜上裂解缓冲液中含有Ca 2+。相比之下,慢性粒细胞性白血病(CML)患者的红细胞膜含有67 Kd蛋白,即使在
Cytosolic and membrane fractions of human erythrocytes were probed with antisera raised against several members of the annexin family of Caz+-dependent phospholipid/ membrane-binding proteins. One of the antisera, that against the 67 Kd calcimedin, identified erythrocyte polypeptides of molecular weights 48 and 67 Kd, which were found in the cytoplasm when normal erythrocytes were lysed in the presence of EGTA but on the membrane when lysis buffers contained Ca2+. In contrast, membranes of erythrocytes from patients with chronic myelogenous leukemia (CML) contained the 67 Kd protein even when
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DOI: 10.1016/0309-1651(85)90051-7
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