Presclerotized eggshell protein from the liver fluke Fasciola hepatica.
Presclerotized eggshell protein from the liver fluke Fasciola hepatica.
复制标题
来自肝片形吸虫的预硬化蛋壳蛋白。
DOI：
10.1021/bi00398a043
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Rice-Ficht,AC
中科院分区：
文献类型：
--
作者：
Waite,JH;Rice-Ficht,AC
医学生物化学和遗传学系，得克萨斯A&M大学，学院站，得克萨斯77843接收1987年5月13日;修订后的Mandarin pt接收1987年6月26日摘要：吸虫寄生虫保护他们的鸡蛋与一个坚韧的晒黑的蛋壳。蛋壳前体蛋白在动物广泛的卵黄中合成和储存。从肝片吸虫卵黄中分离出一种主要的蛋壳前体蛋白，其表观分子量为31000，p/为7.4。这种蛋白质占成熟片形吸虫总蛋白质的6-7%，其独特之处在于含有相当高水平的氨基酸3，4-二羟基苯丙氨酸（DOPA），即每1000个氨基酸中含有110个残基。其他主要的氨基酸是甘氨酸、天冬氨酸和赖氨酸。一个主要的含DOPA的胰蛋白酶肽衍生自蛋壳前体蛋白，其序列为Gly-Gly-Gly-DOPA-Gly-Gly-DOPA-Gly-Lys。多巴残留消失在成熟的蛋壳和蘑菇多酚氧化酶在体外处理。这种消失可能与蛋壳蛋白中交联的形成有关。肝吸虫肝片吸虫是一种寄生吸虫，在世界各地发生，并导致感染绵羊的“肝腐”和牛、山羊和人类的轻度消化道并发症。未成熟的片形吸虫通过肝实质移动，消耗大量的组织。片形吸虫成熟后，从肝实质移到肝脏的胆管，在那里它以血液为食，留下一串卵和粪便。一只成熟的肝吸虫平均每天产2500个卵（Bjorkman & Thorsell，1963）。其中一些卵进入肠道，通过肠道继续它们的生命周期。然而，包埋的卵最终会分解，在周围组织中引起严重的炎症和纤维化反应（Pantelouris，1965; Malek，1980）。
Department of Medical Biochemistry and Genetics, Texas A&M University, College Station, Texas 77843 Received May 13, 1987; Revised Manuscript Received June 26, 1987 abstract: Trematode parasites protect their eggs with a tough tanned eggshell. Eggshell precursor proteins are synthesized and stockpiled within the extensive vitellaria of the animal. A major eggshell precursor protein with an apparent molecular weight of 31000 and p/of 7.4 was isolated from the vitellaria of Fasciola hepatica. This protein, which represents 6-7% of the total protein in mature Fasciola, is unique in containing rather high levels of the amino acid 3, 4-dihydroxyphenylalanine (DOPA), ie, 110 residues per 1000. Other prominent amino acids are glycine, aspartic acid, and lysine. A prominent DOPA-containingtryptic peptide derived from eggshell precursor protein has the sequence Gly-Gly-Gly-DOPA-Gly-Gly-DOPA-Gly-Lys. DOPA residues disappear during the maturation of the eggshell and by treatment in vitro with mushroom polyphenol oxidase. This disappearance may be related to the formation of cross-links in the eggshell protein. e liver fluke Fasciola hepatica is a parasitic trematode that occurs throughout the world and causes “liver rot” in infected sheep and milder cirrhotic complications in cattle, goats, and humans. Immature Fasciola migrates through the liver parenchyma, consumingcopious amounts of tissue. Upon maturation, Fasciola moves from the parenchyma to the bile ducts of the liver where it feasts on blood, leaving a trail of eggs and fecalmaterial. A mature liver fluke produces an average of 2500 eggs daily (Bjorkman & Thorsell, 1963). Some of these eggs find their way into the gut, through which they are passed to continue theirlife cycles. The entrapped eggs, however, eventually decompose, setting up serious in-flammatory and fibrotic reactions in surrounding tissues (Pantelouris, 1965; Malek, 1980).