Composition and variation of respiratory microbiota in healthy military personnel
Composition and variation of respiratory microbiota in healthy military personnel
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DOI：
10.1371/journal.pone.0188461
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发表时间：
2017-12-07
期刊：
影响因子：
3.7
通讯作者：
Keiser, Paul B.
中科院分区：
文献类型：
--
作者：
Hang, Jun;Zavaljevski, Nela;Keiser, Paul B.
某些职业和地理暴露与肺部疾病风险增加有关。作为未来研究的基线，我们试图描述驻军环境中健康军事人员上呼吸道微生物组的特征。在一年内从 50 名健康现役志愿者身上收集了 8 次鼻、口咽和鼻咽拭子（1107 份拭子，完成率 = 92.25%），并对 16S rDNA V1-V3 区域进行焦磷酸测序。使用 QIIME 1.8 和核糖体数据库项目分类器在属水平上对呼吸道细菌分类群进行了表征。在鼻腔和鼻咽部微生物群中观察到高水平的葡萄球菌、棒状杆菌和丙酸杆菌，占所有操作分类单位 (OTU) 的 75% 以上。相比之下，链球菌是口咽部唯一的优势细菌属（约占所有 OTU 的 50%）。在所有时间点，口咽部的平均细菌多样性均高于鼻部或鼻咽部。多样性分析表明鼻腔和鼻咽样本之间存在显着重叠，而口咽样本形成了与这两个区域不同的簇。该研究针对健康现役军人呼吸道微生物组的细菌 16S rDNA V1-V3 区域生成了大量焦磷酸测序数据。测序读数的预处理显示出良好的数据质量。得出的微生物组概况在内部和之前的报告中均一致，表明它们可用于基于序列和人口统计数据的进一步分析和关联研究。
Certain occupational and geographical exposures have been associated with an increased risk of lung disease. As a baseline for future studies, we sought to characterize the upper respiratory microbiomes of healthy military personnel in a garrison environment. Nasal, oropharyngeal, and nasopharyngeal swabs were collected from 50 healthy active duty volunteers eight times over the course of one year (1107 swabs, completion rate = 92.25%) and subjected to pyrosequencing of the V1-V3 region of 16S rDNA. Respiratory bacterial taxa were characterized at the genus level, using QIIME 1.8 and the Ribosomal Database Project classifier. High levels of Staphylococcus, Corynebacterium, and Propionibacterium were observed among both nasal and nasopharyngeal microbiota, comprising more than 75% of all operational taxonomical units (OTUs). In contrast, Streptococcus was the sole dominant bacterial genus (approximately 50% of all OTUs) in the oropharynx. The average bacterial diversity was greater in the oropharynx than in the nasal or nasopharyngeal region at all time points. Diversity analysis indicated a significant overlap between nasal and nasopharyngeal samples, whereas oropharyngeal samples formed a cluster distinct from these two regions. The study produced a large set of pyrosequencing data on the V1-V3 region of bacterial 16S rDNA for the respiratory microbiomes of healthy active duty Service Members. Pre-processing of sequencing reads showed good data quality. The derived microbiome profiles were consistent both internally and with previous reports, suggesting their utility for further analyses and association studies based on sequence and demographic data.