High molecular weight hyaluronan decreases UVB-induced apoptosis and inflammation in human epithelial corneal cells
High molecular weight hyaluronan decreases UVB-induced apoptosis and inflammation in human epithelial corneal cells
复制标题
DOI：
--
复制
发表时间：
2009-03
期刊：
影响因子：
2.2
通讯作者：
Thierry Pauloin;M. Dutot;F. Joly;J. Warnet;P. Rat
中科院分区：
文献类型：
--
作者：
Thierry Pauloin;M. Dutot;F. Joly;J. Warnet;P. Rat
目的研究高分子量透明质酸（HMW-HA）对人角膜上皮细胞（HCE）抗紫外线B （UVB）辐射毒性的保护作用。方法用HMW-HA或磷酸盐缓冲盐溶液（PBS）培养HCE细胞株，冲洗，UVB照射。评估细胞活力、活性氧（ROS）和谷胱甘肽（GSH）水平、8-羟基-2′-脱氧鸟苷（8-oxo-dG）释放、p53磷酸化、caspase-3、-8、-9活化和白细胞介素(IL)-6和-8的产生，以评估和比较HMW-HA处理细胞和PBS处理细胞之间uvb诱导的毒性。结果HMW-HA对UVB辐射有明显的防护作用。HMW-HA提高了HCE细胞活力，降低了IL-6和-8的产生，降低了caspase-3和-8的活化。然而，HMW-HA对ROS和GSH水平、8-oxo-dG释放和p53磷酸化没有显著影响。据我们所知，我们首次报道了HMW-HA保护细胞免受紫外线照射的能力。根据我们的研究结果，HMW-HA为暴露于UVB的细胞提供抗炎和抗凋亡信号。
Purpose The aim of this study was to investigate high molecular weight hyaluronan (HMW-HA) protection on human corneal epithelial (HCE) cells against ultraviolet B (UVB) radiation-induced toxic effects. Methods The HCE cell line was incubated with HMW-HA or phosphate-buffered salt solution (PBS), rinsed, and exposed to UVB radiation. Cell viability, reactive oxygen species (ROS) and glutathione (GSH) levels, 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) release, p53 phosphorylation, caspase-3, -8, -9 activation, and interleukin (IL)-6 and -8 production were assessed to evaluate and to compare UVB-induced toxicity between cells treated with HMW-HA and cells treated with PBS. Results Data indicate that HMW-HA had significant protective effects against UVB radiation. HMW-HA increased HCE cell viability, decreased IL-6 and -8 production, and decreased caspase-3 and -8 activation. However, HMW-HA had no significant effect on ROS and GSH levels, 8-oxo-dG release, and p53 phosphorylation. Conclusions To our knowledge, we report for the first time the ability of HMW-HA to protect cells against UV irradiation. According to our results, HMW-HA provides anti-inflammatory and anti-apoptotic signals to cells exposed to UVB.