EVIDENCE THAT SPITZENKORPER BEHAVIOR DETERMINES THE SHAPE OF A FUNGAL HYPHA - A TEST OF THE HYPHOID MODEL
EVIDENCE THAT SPITZENKORPER BEHAVIOR DETERMINES THE SHAPE OF A FUNGAL HYPHA - A TEST OF THE HYPHOID MODEL
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DOI：
10.1006/emyc.1995.1017
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发表时间：
1995-06-01
期刊：
影响因子：
--
通讯作者：
BRACKER, CE
中科院分区：
文献类型：
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作者：
BARTNICKIGARCIA, S;BARTNICKI, DD;BRACKER, CE
立枯丝核菌的菌丝在其生长尖端具有特征性的Spitzenkorper，并且细胞形状由数学菌丝体方程描述。一个轻微的干扰菌丝生长在载玻片培养室的显微镜阶段导致Spitzenkorper移动远离其通常的位置旁边的顶端极和徘徊短暂的顶端圆顶内。菌丝伸长速率急剧下降，顶端变圆，直径增大。随着Spitzenkorper迁移回其极性位置，细胞迅速伸长恢复，和轮廓的生长菌丝顶端恢复到典型的菌丝形状。Spitzenkorper的短暂错位在菌丝轮廓中留下了永久性的隆起。这一形态发生序列是通过计算机模拟的基础上，菌丝方程，其中涉及到一个囊泡供应中心（VSC）的线性位移的菌丝形状的产生。VSC被编程为在上述序列中回溯观察到的Spitzenkorper的运动。真实的和计算机模拟的细胞之间的形状的相似性加强了数学预测，即Spitzenkorper作为一个VSC，其连续的线性前进产生一个典型的菌丝管与特征的菌丝体形状。因此，菌丝体模型及其VSC概念提供了一个合理的假说来解释真菌菌丝极化生长的细胞基础。(C)出版社：Academic Press
Hyphae of the fungus Rhizoctonia solani have a characteristic Spitzenkorper in their growing tips and a cell shape described by the mathematical hyphoid equation. A mild disturbance of hyphae growing in a slide culture chamber on a microscope stage caused the Spitzenkorper to move away from its usual position next to the apical pole and wander briefly inside the apical dome. Hyphal elongation rate declined abruptly, and the apex became rounded and increased in diameter. As the Spitzenkorper migrated back to its polar position, rapid cell elongation resumed, and the contour of the growing hyphal tip returned to the typical hyphoid shape. The brief dislocation of the Spitzenkorper left a permanent bulge in the hyphal profile. This morphogenetic sequence was mimicked by computer simulation, based on the hyphoid equation which relates the generation of hyphal shape to the linear displacement of a vesicle supply center (VSC). The VSC was programmed to retrace the observed movements of the Spitzenkorper during the above sequence. The resulting similarity of shape between real and computer-simulated cells reinforces the mathematical prediction that the Spitzenkorper acts as a VSC and that its continuous linear advancement generates a typical hyphal tube with the characteristic hyphoid shape. Accordingly, the hyphoid model and its VSC concept provide a plausible hypothesis to explain the cellular basis of polarized growth of fungal hyphae. (C) 1995 Academic Press, Inc.