Immunoglobulin µ and γ heavy chain classes are not determined by class-specific RNA-splicing enzymes
Immunoglobulin µ and γ heavy chain classes are not determined by class-specific RNA-splicing enzymes
复制标题
免疫球蛋白 µ 和 γ 重链类别不是由类别特异性 RNA 剪接酶决定的
作者：
M. Shulman;G. Köhler
产生免疫球蛋白 (Ig) 的淋巴细胞有时可以表达一个重链可变 (VH) 区与不同的重链恒定 (CH) 区。例如，已经观察到产生具有相同抗体特异性的 IgM 和 IgD 的单细胞1。细胞，无论是单独2还是在大规模培养中3,4，都会从IgM生产转变为IgG生产，并且据信表达的可变区在转换之前和之后是相同的。我们在这里描述了对通过将 IgM 产生细胞与 IgG 产生者融合而产生的杂交体的分析，其中相应的 μ 和 γ 重链具有不同的可变区，表示为 V1 和 V2。这种杂交只产生亲本重链——V1Cμ和V2Cγ——而不是新的组合V2Cμ。这一结果表明 μ 和 γ 重链类别不是由类别特异性 RNA 加工决定的。
An immunoglobulin (Ig)-producing lymphocyte can sometimes express one heavy-chain variable (VH) region combined with different heavy-chain constant (CH) regions. For example, single cells making IgM and IgD with the same antibody specificity have been observed1. Cells, both singly2 and in mass culture3,4, switch from IgM to IgG production, and it is believed that the variable regions expressed are the same before and after the switch. We describe here the analysis of a hybrid derived by fusing an IgM-producing cell with an IgG producer, where the corresponding μ, and γ heavy chains bear different variable regions, denoted V1 and V2. This hybrid makes only the parental heavy chains—V1Cμ and V2Cγ—and not the novel combination V2Cμ. This result suggests that the μ and γ heavy-chain classes are not determined by classspecific RNA processing.