NADPH oxidases in the arbuscular mycorrhizal symbiosis
NADPH oxidases in the arbuscular mycorrhizal symbiosis
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DOI：
10.1080/15592324.2016.1165379
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发表时间：
2016-01-01
影响因子：
2.9
通讯作者：
Lanfranco, Luisa
中科院分区：
文献类型：
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作者：
Belmondo, Simone;Calcagno, Cristina;Lanfranco, Luisa
植物NADPH氧化酶是植物体内活性氧(ROS)的主要来源，ROS在植物对病原菌的防御反应等过程中起着信号和胁迫的重要作用。在丛枝菌根(AM)发育过程中根细胞中ROS的积累引起了人们对理解ROS介导的防御程序在这种相互作用的建立过程中是如何被调节的兴趣。我们最近分析了5个NADPH氧化酶(也称为RBOH)编码基因的表达模式，结果表明，其中只有一个基因(MtRbohE)在含丛枝的细胞中特异性上调。与此结果一致的是，沉默MtRbohE的RNAi导致了根部定植的强烈变化，丛生细胞的数量显著减少。在此基础上，我们认为MtRBOHE介导的ROS产生在丛生植物的细胞内调节中起着至关重要的作用。
Plant NADPH oxidases are the major source of reactive oxygen species (ROS) that plays key roles as both signal and stressor in several plant processes, including defense responses against pathogens. ROS accumulation in root cells during arbuscular mycorrhiza (AM) development has raised the interest in understanding how ROS-mediated defense programs are modulated during the establishment of this mutualistic interaction. We have recently analyzed the expression pattern of 5 NADPH oxidase (also called RBOH) encoding genes in Medicago truncatula, showing that only one of them (MtRbohE) is specifically upregulated in arbuscule-containing cells. In line with this result, RNAi silencing of MtRbohE generated a strong alteration in root colonization, with a significant reduction in the number of arbusculated cells. On this basis, we propose that MtRBOHE-mediated ROS production plays a crucial role in the intracellular accommodation of arbuscules.