Plants ectopically expressing the iron-binding protein, ferritin, are tolerant to oxidative damage and pathogens
Plants ectopically expressing the iron-binding protein, ferritin, are tolerant to oxidative damage and pathogens
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DOI：
10.1038/6198
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发表时间：
1999-02-01
影响因子：
46.9
通讯作者：
Dudits, D
中科院分区：
文献类型：
--
作者：
Deák, M;Horváth, GV;Dudits, D
在营养组织中合成苜蓿铁蛋白的转基因烟草植物——无论是叶绿体中的加工形式还是细胞质中的未加工形式——在铁过量或百草枯处理产生自由基毒性后保留光合功能。在叶子中积累铁蛋白的转基因植物的后代表现出对病毒（烟草坏死病毒）和真菌（链格孢、灰葡萄孢）感染引起的坏死损伤的耐受性。这些转化体在温室条件下表现出正常的光合功能和叶绿素含量。我们提出，通过芬顿反应螯合细胞内铁，参与产生非常活跃的羟基自由基，铁蛋白可以保护植物细胞免受各种应激引起的氧化损伤。
Transgenic tobacco plants that synthesize alfalfa ferritin in vegetative tissues-either in its processed form in chloroplasts or in the cytoplasmic nonprocessed form-retained photosynthetic function upon free radical toxicity generated by iron excess or paraquat treatment. Progeny of transgenic plants accumulating ferritin in their leaves exhibited tolerance to necrotic damage caused by viral (tobacco necrosis virus) and fungal (Alternaria alternata, Botrytis cinerea) infections. These transformants exhibited normal photosynthetic function and chlorophyll content under greenhouse conditions. We propose that by sequestering intracellular iron involved in generation of the very reactive hydroxyl radicals through a Fenton reaction, ferritin protects plant cells from oxidative damage induced by a wide range of stresses.