Role of neuropsin in formation and maturation of Schaffer-collateral L1cam-immunoreactive synaptic boutons
Role of neuropsin in formation and maturation of Schaffer-collateral L1cam-immunoreactive synaptic boutons
复制标题
DOI：
10.1242/jcs.02862
复制
发表时间：
2006-04-01
影响因子：
4
通讯作者：
Shiosaka, S
中科院分区：
文献类型：
--
作者：
Nakamura, Y;Tamura, H;Shiosaka, S
我们报告说，neuropsin参与突触发育/成熟的孤儿和小突触扣在谢弗侧支通路。大多数非突触孤儿终扣和一些未成熟的小突触终扣表达的细胞粘附分子L1在突触前Schaffer侧支末梢，而成熟的蘑菇刺上的大终扣没有L1。L1免疫反应终结的数量显着高于neuropsin缺陷小鼠比野生型小鼠，而有少得多的成熟的大终结。L1-免疫反应终结在突变小鼠中肥大。当重组活性neuropsin显微注射到突变海马，免疫反应突触扣的数量恢复到野生型水平后一天。这些结果强烈表明，酶活性neuropsin允许成熟的变化L1-免疫反应性的小boutons，孤儿和突触，这一步可能是重要的活动依赖性的结构变化的基础上的突触可塑性。
We report that neuropsin is involved in the synaptogenesis/maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway. Most nonsynaptic orphan boutons and a number of immature small synaptic boutons expressed the cell adhesion molecule L1 in presynaptic Schaffer-collateral terminals, whereas mature large boutons on mushroom spines were devoid of L1. The number of L1-immunoreactive boutons was markedly higher in neuropsin-deficient mice than in wildtype mice, whereas there were far fewer mature large boutons. L1-immunoreactive boutons were hypertrophied in the mutant mice. When a recombinant active neuropsin was microinjected into the mutant hippocampus, the number of immunoreactive synaptic boutons reverted to wild-type levels after one day. These results strongly suggest that enzymatically active neuropsin allows a maturational change of L1-immunoreactive small boutons, both orphan and synaptic, and this step may be important in synaptic plasticity based on activity-dependent structural change.