A magneto-optic route toward the in vivo diagnosis of malaria: Preliminary results and preclinical trial data
A magneto-optic route toward the in vivo diagnosis of malaria: Preliminary results and preclinical trial data
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DOI：
10.1529/biophysj.107.128140
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发表时间：
2008-07-15
影响因子：
3.4
通讯作者：
Mensz, Petra F.
中科院分区：
文献类型：
--
作者：
Newman, Dave M.;Heptinstall, John;Mensz, Petra F.
我们报告了用于疟疾快速定量诊断的磁光技术的发展，该技术也可以以非侵入性形式实现。疟原虫色素是疟疾对血红蛋白寄生作用的废物，其产生形式是在外加磁场的作用下产生疟原虫色素浓度的诱导光学二色性特征。在这里，我们表明，对这种诱导的二色性的精确测量可用于确定疟疾感染的水平，因为它与感染患者血液和组织中疟原虫色素的浓度相关，尽管在整个感染周期中以复杂的方式相关。根据寄生虫血症函数产生疟原虫色素的保守假设，初步结果表明该技术可以匹配或超过其他当前的诊断技术。对 13 名患者进行的小型初步临床试验证实了该方法的有效性，并且对从体外培养获得的活寄生细胞进行的测量验证了生产体内诊断仪器的可能性。
We report the development of magneto-optic technology for the rapid quantitative diagnosis of malaria that may also be realizable in a noninvasive format. Hemozoin, the waste product of malarial parasitic action on hemoglobin, is produced in a form that under the action of an applied magnetic field gives rise to an induced optical dichroism characteristic of the hemozoin concentration. Here we show that precise measurement of this induced dichroism may be used to determine the level of malarial infection because this correlates, albeit in a complex manner throughout the infection cycle, with the concentration of hemozoin in the blood and tissues of infected patients. Under conservative assumptions for the production of hemozoin as a function of parasitemia, initial results indicate that the technique can match or exceed other current diagnostic techniques. The validity of the approach is confirmed by a small preliminary clinical trial on 13 patients, and measurements on live parasitized cells obtained from in vitro culture verify the possibility of producing in vivo diagnostic instrumentation.