DISTRIBUTION OF NEURONS AND GLIA IN THE VISUAL-CORTEX (AREA-17) OF THE ADULT ALBINO-RAT - A QUANTITATIVE DESCRIPTION
DISTRIBUTION OF NEURONS AND GLIA IN THE VISUAL-CORTEX (AREA-17) OF THE ADULT ALBINO-RAT - A QUANTITATIVE DESCRIPTION
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DOI：
10.1016/0306-4522(87)90040-6
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发表时间：
1987-06-01
期刊：
影响因子：
3.3
通讯作者：
STEWART, MG
中科院分区：
文献类型：
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作者：
GABBOTT, PLA;STEWART, MG
用体视学方法对大鼠视皮层（17区）的神经元和神经胶质细胞组成进行了定量测定。神经元和主要神经胶质细胞类型（星形胶质细胞、少突胶质细胞和小胶质细胞）的体积数密度（每mm 3皮质的细胞数）由在整个视觉皮层深度上以规则间隔50 μ m间隔的切向半薄树脂切片计算。从皮质和层厚度的测量，神经元和神经胶质细胞的单独的体积数密度推导出每个层在皮质。此外，计算皮质表面1 mm 2下各层中细胞的绝对数量。神经元的平均皮质体积数密度为60，020 ± 0.001。3840/mm ~ 3（.hivin.X . ±. SEM; n=8）和49，040. ±-. 2610/mm 3的组合胶质细胞类型。星形胶质细胞、少突胶质细胞和小胶质细胞分别以6：3：1的比例存在。从神经元和神经胶质体细胞体积估计确定，这些细胞的胞体占据约13.5%的单位皮质体积，其中81.3%的单位体积被皮质神经元占据。使用以前发表的报告，描述了神经元的锥体细胞和非锥体细胞的相对比例的层状组成，这些神经元类别的层状体积数密度已被导出。此外，据估计，在1平方毫米的皮层表面下，有79，500个锥体神经元和7790个非锥体神经元分布在大鼠视觉皮层的第1-6层。
The neuronal and glial cell composition of the rat visual cortex (area 17) has been determined quantitatively using stereological techniques. The volume numerical densities (number of cells per mm3 of cortex) of neurons and of the principal glial cell types (astroglia, oligodendroglia, and microglia) were calculated from tangential semithin resin sections spaced at regular intervals 50 .mu.m apart throughout the entire depth of the visual cortex. From measurements of cortical and laminar thickness the separate volume numerical densities of neurons and glial cells were derived for each lamina in the cortex. In addition, the absolute numbers of cells in each lamina under 1 mm2 of cortical surface were calculated. The mean cortical volume numerical density of neurons was 60,020 .+-. 3840/mm3 (.hivin.X .+-. SEM; n=8), and 49,040 .+-. 2610/mm3 for the combined glial cell types. Astroglia, oligodendroglia, and microglia were present in a ratio of 6:3:1 respectively. It was determined from neuronal and glial somatic volume estimates that the somata of these cells occupied approximately 13.5% of unit cortical volume, with 81.3% of the unit volume being occupied by cortical neuropil. Using previously published reports that described the laminar composition of neurons in terms of the relative proportions of pyramidal and non-pyramidal cells, the laminar volume numerical densities for these neuronal categories have been derived. In addition, it has been estimated that under 1 mm2 of cortical surface there are 79,500 pyramidal and 7790 non-pyramidal neurons distributed throughout layers 1-6 of the rat visual cortex.