Multiphase method for automatic alignment of transmission electron microscope images using markers
Multiphase method for automatic alignment of transmission electron microscope images using markers
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DOI：
10.1006/jsbi.2001.4343
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发表时间：
2001-01-01
影响因子：
3
通讯作者：
Engelhardt, P
中科院分区：
文献类型：
--
作者：
Brandt, S;Heikkonen, J;Engelhardt, P
为了成功地进行电子断层扫描的三维重建，透射电子显微镜图像必须准确地对齐或配准。到目前为止，通过手动显示来自图像集合的对应基准标记或自动使用几个旋转和尺度上的图像之间的简单相关性来解决该问题。然而，目前的解决方案都存在效率低和/或不准确的问题。因此，我们提出了一种方法，其中注册是自动化的，使用传统的胶体金颗粒作为参考标记之间的图像。我们从计算机视觉的角度来处理这个问题;因此，对准问题被分为几个子问题：（1）从连续图像中找到初始匹配，（2）估计连续图像之间的对极几何形状，（3）在每个图像中以亚像素精度找到并定位金颗粒，（4）使用对极约束及其不确定性预测可能的匹配金颗粒，（5）通过倾斜序列匹配和跟踪金珠，以及（6）优化整个图像集的变换参数。结果表明，不仅建议的方法的可靠性，但也在对齐的高精度水平，因为几乎所有的可见金标记可以使用。(C)北京：科学出版社.
In order to successfully perform the 3D reconstruction in electron tomography, transmission electron microscope images must be accurately aligned or registered. So far, the problem is solved by either manually showing the corresponding fiducial markers from the set of images or automatically using simple correlation between the images on several rotations and scales. The present solutions, however, share the problem of being inefficient and/or inaccurate. We therefore propose a method in which the registration is automated using conventional colloidal gold particles as reference markers between images. We approach the problem from the computer vision viewpoint; hence, the alignment problem is divided into several subproblems: (1) finding initial matches from successive images, (2) estimating the epipolar geometry between consecutive images, (3) finding and localizing the gold particles with subpixel accuracy in each image, (4) predicting the probable matching gold particles using the epipolar constraint and its uncertainty, (5) matching and tracking the gold beads through the tilt series, and (6) optimizing the transformation parameters for the whole image set. The results show not only the reliability of the suggested method but also a high level of accuracy in alignment, since practically all the visible gold markers can be used. (C) 2001 Academic Press.