Endogenous abscisic acid signaling towards storage reserve filling in developing seed tissues of castor bean (Ricinus communis L.)
Endogenous abscisic acid signaling towards storage reserve filling in developing seed tissues of castor bean (Ricinus communis L.)
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DOI：
10.1007/s10725-013-9846-z
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发表时间：
2014-03-01
影响因子：
4.2
通讯作者：
Liu, Aizhong
中科院分区：
文献类型：
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作者：
Chandrasekaran, Umashankar;Liu, Aizhong
脱落酸(ABA；游离态)是高等植物中一种天然存在的生理生长激素。用一种新的酶联免疫吸附试验对种子组织发育过程中与内源游离ABA水平相关的生长进行了详细的研究。蓖麻种子灌浆的研究胚乳、胚和豆荚在发育中期[授粉后21-42天]鲜重迅速增加，在成熟期(42-63天)鲜重稳步下降，同时伴随着快速的脂肪合成(胚乳和胚)，但在豆荚中除外。胚乳(0.001~0.32mU g/g)和胚(0.003~0.13mU g/g)的内源ABA水平与种子储备灌浆呈平行关系，在发育中期(21~42DAP)呈快速上升趋势，而豆荚(0.2mU/g~22.9mU/g)则表现出不同的积累模式，在发育早期快速上升和下降，成熟期(63DAP)达到最大值。综上所述，我们的结果为内源ABA在种子灌浆和储备积累中的关联提供了证据，并为外源ABA浓度在种子发育中的有效利用提供了线索，重点是改善蓖麻的种子储备复合体。
Abscisic acid (ABA; free form) is a naturally occurring physiological growth hormone of higher plants. A detailed study involving the time course growth of developing seed tissues associated with endogenous levels of free ABA were investigated using a novel enzyme-linked immunosorbent assay. Seed filling in castor (Ricinuc communis L.) endosperm, embryo, and pod is marked with a rapid increase in fresh weight during the mid-developmental stages [21-42 days after pollination (DAP)], followed by a steady decline at the maturation stages (42-63 DAP) accompanied with a rapid lipid synthesis (in endosperm and embryo) during the same period, except for in pod. Endogenous ABA levels in endosperm (0.001-0.32 mu g/g) and embryo (0.003-0.13 mu g/g) followed a concurrent pattern with seed reserve filling, showing a rapid increase during the mid-developmental stages 21-42 DAP, whereas ABA levels in seed pod (0.2-22.9 mu g/g) showed a different accumulation pattern with rapid increase and decline during the early-mid developmental stages, preceded by the maximal increase during the maturation stage (63 DAP). Together, our results provide evidence for the association of endogenous ABA in seed filling as well as in reserve deposition and provides clue for the effective usage of exogenous ABA concentrations in developing seeds with a focus, on improving seed reserve complex in castor.