Differential gene expressions and phytohormone changes altered Lonicera japonica quality after plant introduction
Differential gene expressions and phytohormone changes altered Lonicera japonica quality after plant introduction
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引入植物后差异基因表达和植物激素变化改变了忍冬的品质
DOI：
10.4103/pm.pm_317_18
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发表时间：
2019-01-01
影响因子：
0.7
通讯作者：
Ou, Chunli
中科院分区：
文献类型：
--
作者：
Chen, Xiaoying;Qin, Shuangshuang;Ou, Chunli
背景：日本野牡丹（Escherajaponica Thunb.）(LJ)是一种著名的传统中药植物。由于其巨大的生命力和重要的药用价值，LJ从原产地被引入非原产地。然而，LJ植物生产后的质量变化仍不清楚。目的：本研究的目的是分析生物活性物质，相应的基因表达，以及内源植物激素的变化后，3年的引进。材料与方法：2014年9月，在两个地点种植LJ插条。2017年5月，随机收获LJ花蕾，分为三组，分别进行液相色谱-串联质谱（LC-MS/MS）、实时定量逆转录-聚合酶链反应（RT-PCR）和间接酶联免疫吸附试验（ELISA）。结果如下：结果表明，引种后绿原酸含量显著降低，而木犀草苷和木犀草素含量显著增加。与生物合成途径相关的基因表达谱显示差异表达模式，尽管其中大多数没有显着差异。羟基肉桂酰辅酶A奎尼酸羟基肉桂酰转移酶基因表达下调导致绿原酸含量下降，而与合成通用前体p-香豆酰辅酶A和分支木犀草苷生物合成途径相关的关键基因表达上调导致木犀草苷含量增加。改变内源激素水平，特别是茉莉酸，调节LJ质量的变化。结论：数据表明，无论绿原酸的减少，黄酮类化合物的显着增加证明了LJ引入的好处。所用缩写：LJ：日本芹; PAL：苯丙氨酸解氨酶（EC 4.3.1.24）; C4 H：肉桂酸4-羟化酶（EC 1.14.13.11）; 4CL：4-羟基肉桂酰-CoA连接酶/4-香豆酸-CoA连接酶（EC 6.2.1.12）; HQT：羟基肉桂酰-CoA奎尼酸羟基肉桂酰转移酶（EC 2.3.1.99）; CHS：查耳酮合酶（EC 2.3.1.74）; CHI：查耳酮异构酶（EC 1.14.11.22）; FNS：黄酮合酶（EC5.5.1.6）; GL：糖基转移酶（黄酮7-O-β-葡糖基转移酶，EC 2.4.1.81）。
Background: Lonicera japonica Thunb. (LJ) is a famous traditional Chinese medicinal plant. For great viability and important medical value, LJ was introduced from the geo-authentic areas to nonauthentic production areas. However, the quality changes in LJ after plant production remained unclear. Objective: The objective of the study is to analyze the bioactive compounds, the corresponding gene expressions, and the endogenous plant hormone changes after 3-year introduction. Materials and Methods: In September 2014, LJ cuttings were planted in the two sites. In May 2017, LJ flower buds were randomly harvested and divided into three groups for liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), quantitative real-time reverse transcription-polymerase chain reaction, and indirect enzyme-linked immunosorbent assay, respectively. Results: The results showed that chlorogenic acid significantly decreased while luteoloside and luteolin significantly increased after plant introduction. Gene expression profiles related to biosynthesis pathways showed differentially expressed patterns although most of them were not significantly different. The down-regulated expression of hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase genes resulted in a decrease of chlorogenic acid content while the up-regulated expression of key genes related to synthesizing the universal precursor p-Coumaroyl-CoA and the branched luteoloside biosynthetic pathway led to an increase of luteoloside content. Altered endogenous phytohormone levels, especially jasmonic acid, regulate LJ quality changes. Conclusion: The data demonstrate that irrespective of the decrease of chlorogenic acid, the considerable increase of flavonoids demonstrated the benefit of LJ introduction. Abbreviations used: LJ: Lonicera japonica Thunb.; PAL: Phenylalanine ammonia-lyase (EC 4.3.1.24); C4H: Cinnamate 4-hydroxylase (EC 1.14.13.11); 4CL: 4-Hydroxycinnamoyl-CoA ligase/4-coumarate-CoA ligase (EC 6.2.1.12); HQT: Hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (EC 2.3.1.99); CHS: Chalcone synthase (EC 2.3.1.74); CHI: Chalcone isomerase (EC 5.5.1.6); FNS: Flavone synthase (EC 1.14.11.22); GL: Glycosyltransferase (flavone 7-O-beta-glucosyltransferase, EC 2.4.1.81).