Improved technique of heterotopic cervical heart transplantation in mice
Improved technique of heterotopic cervical heart transplantation in mice
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DOI：
10.1097/00007890-199712150-00017
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发表时间：
1997-12-15
期刊：
影响因子：
6.2
通讯作者：
Yasui, H
中科院分区：
文献类型：
--
作者：
Tomita, Y;Zhang, QW;Yasui, H
背景资料。小鼠带血管心脏移植的方法已被描述使用缝合和袖带技术。技术问题限制了它的广泛使用。在这里，我们描述了我们的改良的带袖套技术的宫颈异位移植。通过使用较小的Teflon袖带(外径0.6 mm，内径0.4 mm)和超细尖钳，可以直接拉出颈动脉的边缘，并将动脉的近端越过袖带。同样，用这种直接牵引法可以很容易地在22号袖带上外翻颈外静脉。通过这些改进，缩短了手术时间。供体采集通常需要20min，颈部血管准备通常需要15min，吻合术通常需要15min。从供体采集到受体小鼠皮肤闭合的所有过程都可以在1小时内完成，缺血时间在25-40分钟内。该方法可用于研究环磷酰胺诱导的耐受及再灌注损伤的机制。
Background. The method for mouse vascularized heart transplantation have been described using suture and cuff techniques. Technical problems have limited its widespread use. Here, we describe our method of modified cervical heterotopic transplantation with the cuff technique.Methods. By using a smaller Teflon cuff (external diameter 0.6 mm, internal diameter 0.4 mm) and superfine-tip forceps, it became possible to directly pull the edge of the carotid artery and evert the proximal end of the artery over the cuff. Similarly, the external jugular vein could be easily everted over a 22-gauge cuff with this direct pulling method.Results. By these modifications, the operation time was reduced. It usually takes 20 min for the donor harvest, 15 min for preparation of the cervical vessels, and 15 min for anastomosis. All procedures from the donor harvest through skin closure of the recipient mice can be completed within 1 hr, and ischemic time is within 25-40 min.Conclusions. This method can be used to investigate cyclophosphamide-induced tolerance and mechanisms of reperfusion injury.