Radioprotective effects of dimethyl sulfoxide in golden hamster embryo cells exposed to gamma rays at 77 K. II. Protection from lethal, chromosomal, and DNA damage.
Radioprotective effects of dimethyl sulfoxide in golden hamster embryo cells exposed to gamma rays at 77 K. II. Protection from lethal, chromosomal, and DNA damage.
复制标题
二甲亚砜对 77 K 伽马射线照射的金黄仓鼠胚胎细胞的辐射防护作用。
DOI：
10.2307/3577697
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发表时间：
1990
影响因子：
3.4
通讯作者：
T. Miyazaki
中科院分区：
文献类型：
--
作者：
M. Watanabe;M. Suzuki;K. Suzuki;Y. Hayakawa;T. Miyazaki
将金仓鼠胚胎细胞在存在或不存在二甲亚砜的情况下，在 310 K 和 77 K 下暴露于 137Cs 伽马射线。二甲亚砜在 310 K 和 77 K 下均能显着防止细胞死亡。在 77 K 下使用 1.28 M 二甲亚砜的辐射防护程度是在 310 K 下不存在二甲亚砜时观察到的致死效果的 85-89%；剂量修改因子为5.7。二甲亚砜还可以防止伽马射线诱导的 DNA 单链断裂和染色体畸变，在 77 K 下二甲亚砜浓度为 1.28 M 时，最大保护率为 80-100%。在 77 K 下，H 原子、离子空穴和电子可以通过冷冻细胞迁移，但 OH 自由基不能扩散。因此，二甲基亚砜在 77 K 时对细胞杀伤、染色体畸变和 DNA 单链断裂的保护作用可能是由于清除了 H 原子或其他离子，而不是 OH 自由基。
Golden hamster embryo cells were exposed to 137Cs gamma rays in the presence or absence of dimethyl sulfoxide at both 310 and 77 K. Dimethyl sulfoxide gave significant protection against cell killing at both 310 and 77 K. The extent of radioprotection with 1.28 M dimethyl sulfoxide at 77 K was 85-89% of the lethal effects observed in the absence of dimethyl sulfoxide at 310 K; the dose-modifying factor was 5.7. Dimethyl sulfoxide also exerted protected against gamma-ray-induced DNA single-strand breaks and chromosomal aberrations with a maximum protection of 80-100% at a dimethyl sulfoxide concentration of 1.28 M at 77 K. At 77 K, H atoms, ion holes, and electrons can migrate through frozen cells but OH radicals cannot diffuse. Thus the protective effects of dimethyl sulfoxide against cell killing, chromosomal aberrations, and DNA single-strand breaks at 77 K may be due to the scavenging of H atoms or other ions, rather than OH radicals.