Solid-phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid-based silica sorbent.
Solid-phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid-based silica sorbent.
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DOI：
10.1002/jssc.200900497
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发表时间：
2009-12
影响因子：
3.1
通讯作者：
M. Tian;W. Bi;K. Row
中科院分区：
文献类型：
--
作者：
M. Tian;W. Bi;K. Row
利用合成的离子液体对工业二氧化硅进行表面化学改性，研制出一种新型的离子液体基二氧化硅吸附剂。将制得的甘草酸颗粒成功地用作固相萃取分离甘草中甘草酸和甘草苷的专用吸附剂。考察了不同的洗脱溶剂，如水、甲醇/水(v/v)和纯甲醇。离子液体基二氧化硅吸附剂与传统的C(18)吸附剂相比，具有更高的选择性。采用C(18)柱进行定量分析。两种化合物在5×10~(-4)~0.2 mg/mL(r(2)>；0.99)范围内线性关系良好，相对标准偏差为1.0%。测定了市售含甘草中草药中的目标化合物，并利用该吸附剂测定了目标化合物与蛋白质的结合率。
A new ionic liquid-based silica sorbent was developed by a process involving surface chemical modification of commercial silica using a synthesized ionic liquid. The obtained particles were successfully used as a special sorbent in a solid-phase extraction process to isolate liquiritin and glycyrrhizic acid from licorice. Different washing and elution solvents, such as water, methanol/water (v/v), and pure methanol were evaluated. Ionic liquid-based silica sorbent was compared with traditional C(18) sorbent and it exhibited higher selectivity. Quantitative analysis was carried out by using a C(18) column. Good linearities of two compounds were obtained from 5x10(-4) to 0.2 mg/mL (r(2)>0.99) with the relative standard deviations <1.0%. The target compounds in commercial herbal medicines containing licorice were determined, and the bound rates between the target compounds and protein were obtained by this sorbent.