Phenolic profiling of various olive bark-types and leaves: HPLC–ESI/MS study
Phenolic profiling of various olive bark-types and leaves: HPLC–ESI/MS study
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DOI：
10.1016/j.indcrop.2015.01.077
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发表时间：
2015-05
影响因子：
5.9
通讯作者：
G. Tóth;Ágnes Alberti;A. Sólyomváry;Csenge Barabás;I. Boldizsár;B. Noszál
中科院分区：
文献类型：
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作者：
G. Tóth;Ágnes Alberti;A. Sólyomváry;Csenge Barabás;I. Boldizsár;B. Noszál
油橄榄的酚类成分完整。（橄榄）树皮被周皮或皱皮覆盖的情况首次被确定。为了鉴定化合物，使用了通过LC和电喷雾电离（ESI）与飞行时间（TOF）MS获得的精确分子量和分子式以及通过LC-ESI/MS/MS分析给出的裂解模式。通过经验证的LC-MS方法在MRM模式下使用外标法校准方法对化合物进行定量。结果也进行了定性和定量比较与橄榄叶的酚类成分。我们的分析平台能够表征41种不同的酚类化合物，包括5种羟基香豆素，16种开环环烯醚萜，3种肉桂酸衍生物，8种黄酮类化合物和9种低分子量酚。其中8个化合物为首次从橄榄组织中鉴定。定量分析结果表明，在周苞、皱苞和叶中，化合物的含量有显著差异。老的类型，rhytidome树皮含有大量的羟基香豆素escuaoke（3.01%），而年轻的，perididomite树皮含有微量（0.34%）。该化合物在橄榄叶提取物中根本检测不到。相比之下，裂环烯醚萜类橄榄苦苷在叶中的浓度最高（8.52%），其次是多环烯醚萜（4.98%）和皱皮苷（1.89%）。我们的研究结果清楚地表明，这两种类型的ofO。欧洲树皮是具有药理学和化妆品前景的酚类化合物的廉价、容易获得的天然来源。
The complete phenolic composition ofOlea europaeaL. (olive) barks covered by periderm or rhytidome was determined for the first time. For the identification of compounds accurate molecular mass and formula, acquired by LC and electrospray ionization (ESI) with time-of-flight (TOF) MS and fragmentation pattern given by LC–ESI/MS/MS analyses were used. Quantitation of the compounds was performed by a validated LC–MS method in MRM mode using external standard calibration method. Results were also compared qualitatively and quantitatively with the phenolic composition of olive leaves. Our analytical platform was able to characterize 41 different phenolics including 5 hydroxycoumarins, 16 secoiridoids, 3 cinnamic acid derivatives, 8 flavonoids and 9 low molecular weight phenols. 8 of the compounds were identified for the first time from olive tissues. Quantitative analyses show that the amounts of compounds are significantly different in the periderm, rhytidome and leaf samples. The old type, rhytidome bark contains large amounts of hydroxycoumarin esculetin (3.01%) while the young, periderm bark contains this in traces (0.34%). This compound cannot be detected in olive leaf extract at all. In contrast, the secoiridoid oleuropein is present in the highest concentration in the leaves (8.52%), followed by the periderm (4.98%) and rhytidome (1.89%). Our results clearly show that both types ofO. europaeaL. bark are inexpensive, easily available natural sources of phenolic compounds with pharmacologic and cosmetic perspectives.