Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
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DOI：
10.1084/jem.177.4.1199
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发表时间：
1993-04-01
期刊：
影响因子：
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通讯作者：
Romagnani S
中科院分区：
文献类型：
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作者：
Manetti R;Parronchi P;Giudizi MG;Piccinni MP;Maggi E;Trinchieri G;Romagnani S
研究了在淋巴细胞大量培养中添加或中和白细胞介素12（IL - 12）对体外培养的抗原特异性T细胞系和T细胞克隆发育的影响。在IL - 12存在的情况下产生的针对户尘螨I组（Der p I）的特异性T细胞系表现出产生IL - 4的能力降低，产生干扰素γ（IFN - γ）的能力增强，并发育成为Der p I特异性CD4⁺T细胞克隆，呈现出T辅助细胞0型（Th0）或Th1型而非Th2型的细胞因子谱。相反，在抗IL - 12抗体存在的情况下产生的纯化蛋白衍生物（PPD）特异性T细胞系表现出产生IL - 4的能力增强，并发育成为PPD特异性CD4⁺T细胞克隆，呈现出Th0型而非Th1型的细胞因子谱。向淋巴细胞大量培养物中添加抗IFN - γ抗体并不能阻止IL - 12对Der p I特异性T细胞系细胞因子分泌谱的影响，但从大量培养物中去除CD16⁺细胞至少可部分抑制这种影响。因此，IL - 12和CD16⁺细胞似乎对产生IL - 4的细胞的发育具有抑制作用，并在促进Th1样反应中起诱导作用。
The effects exerted on the in vitro development of antigen-specific T cell lines and T cell clones by addition or neutralization of interleukin 12 (IL-12) in lymphocyte bulk culture were examined. T cell lines specific for Dermatophagoides pteronyssinus group I (Der p I) derived in the presence of IL-12 exhibited reduced ability to produce IL-4 and increased ability to produce interferon gamma (IFN-gamma), and developed into Der p I-specific CD4+ T cell clones showing a T helper type 0 (Th0)- or Th1-, instead of Th2-, like cytokine profile. In contrast, purified protein derivative (PPD)-specific T cell lines derived in the presence of anti-IL-12 antibody exhibited an increased ability to produce IL-4 and developed into PPD-specific CD4+ T cell clones showing a Th0-, instead of Th1-, like profile. The influence of IL-12 on the cytokine secretion profile of Der p I-specific T cell lines was not prevented by addition to lymphocyte bulk cultures of anti- IFN-gamma antibody, but could be at least partially inhibited by the removal from bulk cultures of CD16+ cells. Thus, IL-12 and CD16+ cells appear to have inhibitory effects on the development of IL-4-producing cells and to play an inductive role in promoting Th1-like responses.