Combined sputum hypermethylation and eNose analysis for lung cancer diagnosis
Combined sputum hypermethylation and eNose analysis for lung cancer diagnosis
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DOI：
10.1136/jclinpath-2014-202414
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发表时间：
2014-08-01
影响因子：
3.4
通讯作者：
Thunnissen, Erik
中科院分区：
文献类型：
--
作者：
Hubers, A. Jasmijn;Brinkman, Paul;Thunnissen, Erik
目的本研究的目的是探讨DNA甲基化分析在痰和呼出气分析的互补，非侵入性的诊断能力在lung cancer.Methods痰标本和呼出气前瞻性收集20例肺癌患者和31例COPD对照组（第1组）。另外18名肺癌患者和8名对照仅收集呼出气作为验证集（集2）。结果痰液和电子鼻中的RASSF1A、细胞珠蛋白、APC、FAM19A4、PHACTR3、3OST 2和PRDM14的DNA超甲基化标记物能够独立地区分肺癌患者和对照组。RASSF1A和3OST 2高甲基化联合检测的敏感性为85%，特异性为74%。eNose的敏感性为80%，特异性为48%。当RASSF1A高甲基化与eNose结合时，肺癌诊断的敏感性增加到100%，特异性为42%。两种方法具有互补性（p
Aims The aim of this study is to explore DNA hypermethylation analysis in sputum and exhaled breath analysis for their complementary, non-invasive diagnostic capacity in lung cancer.Methods Sputum samples and exhaled breath were prospectively collected from 20 lung cancer patients and 31 COPD controls (Set 1). An additional 18 lung cancer patients and 8 controls only collected exhaled breath as validation set (Set 2). DNA hypermethylation of biomarkers RASSF1A, cytoglobin, APC, FAM19A4, PHACTR3, 3OST2 and PRDM14 was considered, and breathprints from exhaled breath samples were created using an electronic nose (eNose).Results Both DNA hypermethylation markers in sputum and eNose were independently able to distinguish lung cancer patients from controls. The combination of RASSF1A and 3OST2 hypermethylation had a sensitivity of 85% with a specificity of 74%. eNose had a sensitivity of 80% with a specificity of 48%. Sensitivity for lung cancer diagnosis increased to 100%, when RASSF1A hypermethylation was combined with eNose, with specificity of 42%. Both methods showed to be complementary to each other (p