Recombinant human erythropoietin stimulates angiogenesis and healing of ischemic skin wounds
Recombinant human erythropoietin stimulates angiogenesis and healing of ischemic skin wounds
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DOI：
10.1097/01.shk.0000133591.47776.bd
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发表时间：
2004-08-01
期刊：
影响因子：
3.1
通讯作者：
Frisina, N
中科院分区：
文献类型：
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作者：
Buemi, M;Galeano, M;Frisina, N
在外科实践中，由于血液供应不足而导致的缺血组织（例如皮瓣边缘）中的伤口愈合仍然是相当大的发病率的来源。充分的组织灌注在伤口愈合中特别重要。我们研究了重组人促红细胞生成素（rHuEPO）对缺血性皮肤创伤模型中创伤愈合的影响。使用了63只Sprague-Dawley大鼠。采用正常切口和H形双皮瓣作为创面模型。动物接受rHuEPO（400 IU/kg）或其溶剂治疗。分别于伤后3、5、10 d处死大鼠，取伤后皮肤组织行免疫组化染色，并检测血管内皮生长因子（VEGF）和胶原蛋白含量。组织学标本的组织转氨酶免疫染色被用作血管标记物，以确定微血管密度的水平。结果显示，与正常切口伤口和缺血对照伤口相比，rHuEPO治疗的缺血伤口中血管内皮生长因子蛋白水平和微血管密度增加。与缺血对照伤口相比，切口伤口和用rHuEPO治疗的缺血伤口中的胶原蛋白含量更高。我们的研究结果表明，促红细胞生成素可能是一种有效的治疗方法，在改善缺血性皮肤伤口愈合。
Wound healing in ischemic tissues such as flap margins due to inadequate blood supply is still a source of considerable morbidity in surgical practice. Adequate tissue perfusion is particularly important in wound healing. We investigated the effects of recombinant human erythropoietin (rHuEPO) on wound healing in an ischemic skin wound model. Sixty-three Sprague-Dawley rats were used. Normal incisional wound and H-shaped double flaps were used as the wound models. Animals were treated with rHuEPO (400 IU/kg) or its vehicle. Rats were killed on different days (3, 5, and 10 days after skin injury) and the wounded skin tissues were used for immunohistochemistry and for analysis of vascular endothelial growth factor content and collagen content. Tissue transglutaminase immunostaining of histological specimens was used as a vascular marker to determine the level of microvessel density. The results showed a higher level of vascular endothelial growth factor protein and an increased microvessel density in ischemic wounds with rHuEPO treatment than the normal incisional wounds and ischemic control wounds. Collagen content was higher in the incisional wounds and in the ischemic wounds with rHuEPO treatment compared with the ischemic control wounds. Our results suggest that erythropoietin may be an effective therapeutic approach in improving healing in ischemic skin wounds.