Genetic alterations of gastric cancer: Comparative genomic hybridization and fluorescence in situ hybridization studies
Genetic alterations of gastric cancer: Comparative genomic hybridization and fluorescence in situ hybridization studies
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DOI：
10.1016/s0165-4608(99)00152-1
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发表时间：
2000-03-01
影响因子：
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通讯作者：
Noh, SM
中科院分区：
文献类型：
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作者：
Koo, SH;Kwon, KC;Noh, SM
导致胃癌发展的基因变化仍然存在争议。本研究采用比较基因组杂交（CGH）技术检测37例胃癌组织中DNA拷贝数的变化，同时采用比较基因组杂交（CGH）技术检测37例胃癌组织中沿着所有染色体的DNA拷贝数变化，并对14例胃癌组织进行C-MYC和TP 53探针的荧光原位杂交（FISH）检测。本研究的目的是鉴定那些含有对胃癌发展重要的基因的染色体区域，并鉴定与肿瘤进展相关的遗传标记。最常见的增益是2 q，7 pq，8 pq，13 q，17 q，18 q和20 pq。最常见的缺失区域是17 p。基因改变的模式是不同的，这取决于是否存在淋巴结转移和组织学类型。8 q的增加和17 p的丢失是CGH变化的最常见特征。然而，在10例可用病例中只有3例（30%）通过FISH显示C-MYC基因扩增。TP 53等位基因缺失2例（50%）。这种差异可能是由于这两个基因的另一种重排，而FISH不能检测到，或其他可能的胃癌发生和淋巴结转移。该区域的基因可能与胃癌的发生和淋巴结转移有关。(C)Elsevier Science Inc. 2000. All rights reserved.
Genetic changes leading to the development of gastric cancers are still in dispute. In the following study, we used comparative genomic hydridization (CGH) to screen for DNA copy number following study, we used comparative genomic hybridization (CGH) to screen for DNA copy number changes along all chromosomes in 37 gastric carcinomas, and fluorescence in situ hybridization (FISH) with the C-MYC and TP53 probes in 14 cases for comparison. The aim of this study was to identify those chromosome regions that contain genes important for the development of gastric carcinomas and to identify genetic markers associated with tumor progression. The most often involved gains were 2q, 7pq, 8pq, 13q, 17q, 18q, and 20pq. The most commonly deleted regions were 17p. The pattern of genetic changes was different depending on the existence of nodal metastasis and histologic types. Gains in 8q and losses in 17p were the most common features of the CGH changes. However, only 3 among the available 10 cases (30%) showed an amplification of the C-MYC gene by FISH. Allelic loss of TP53 was found in 2 of 4 cases (50%). This difference might be due to another rearrangement of these 2 genes which cannot be detected by FISH, or other possible genesis and nodal metastasis of gastric carcinomas. genes in that area may be involved in the tumorigenesis and nodal metastasis of gastric carcinomas. (C) Elsevier Science Inc. 2000. All rights reserved.