The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone-binding protein from that found in lepidopteran moths
The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone-binding protein from that found in lepidopteran moths
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Adelphocoris lineolatus OBP4：支持Mirid信息素结合蛋白与鳞翅目蛾中发现的进化和功能差异
DOI：
10.1111/1744-7917.12919
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发表时间：
2022
期刊：
影响因子：
4
通讯作者：
Zhang Yongjun
中科院分区：
文献类型：
--
作者：
Wang Qian;Li Yujie;Wang Qi;Sun Liang;Zhang Yongjun
信息素结合蛋白（Pheromone-binding proteins，PBPs）在鳞翅目蛾类中已得到广泛研究，但其在半翅目昆虫中的进化和功能尚不清楚。我们以前的研究表明，气味结合蛋白，OBP 4，盲蝽Adelphocoris lineolatus的功能作为一个候选半翅目PBP，但与鳞翅目触角结合蛋白（ABPs），而不是在PBP/一般气味结合蛋白（GOBP）分支聚类。在这项研究中，我们假设半翅目昆虫的PBP的起源和功能可能不同于鳞翅目蛾。为了验证这一假设，我们首先构建了一个系统发育树，从姐妹半翅目和全变态谱系的昆虫OBP，结果表明，无论是OBP 4或其他类型的候选PBP的盲蝽聚在鳞翅目PBP/GOBP分支。利用荧光竞争结合法测定了重组OBP 4蛋白与不同功能基团的植物挥发性物质的结合亲和力。线纹夜蛾性信息素成分结果表明，OBP 4与盲蝽雌成虫引诱剂3-己酮和其他15种具有生物活性的植物挥发物高度结合。最后，我们研究了OBP 4的细胞表达谱在假定的触角感器，与女性。Lineolatus寄主植物位置。荧光原位杂交和免疫细胞化学标记分析表明，OBP 4基因在多孔嗅感器、中长锥形感器中高表达，而在短锥形感器和单孔刺形感器中表达较低。这些结果与我们以前的研究结果一起表明，OBP 4不仅在识别昆虫产生的性信息素中起作用，而且可能参与检测寄主植物挥发性物质。lineolatus性别我们的研究结果支持的假设，即在半翅目错误的PBP的起源和功能不同于那些著名的PBP在鳞翅目蛾，这提供了一个新的角度跨昆虫性信息素通信的进化机制。
Pheromone-binding proteins(PBPs)have been extensively investigated in lepidopteran moths, but their evolution and function in hemipteran species remain unclear. Our previous study demonstrated that an odorant-binding protein, OBP4, of the mirid bug Adelphocoris lineolatus functions as a candidate hemipteran PBP but clustered with lepidopteran antennae-binding proteins(ABPs)rather than in the PBP/general odorantbinding protein(GOBP)clade. In this study, we hypothesized that origin and function of PBPs in hemipteran bugs may differ from those of lepidopteran moths. To test this hypothesis, we first constructed a phylogenetic tree using insect OBPs from sister hemipteran and holometabolous lineages, and the results indicated that neither OBP4 nor other types of candidate PBPs of mirid bugs clustered with the lepidopteran PBP/GOBP clade. Then, a fluorescence competitive binding assay was employed to determine binding affinities of recombinant OBP4 protein to host plant volatiles, with functional groups different from A. lineolatus sex pheromone components. The results revealed that OBP4 highly bound the female adult attractant 3-hexanone and 15 other mirid bug biologically active plant volatiles. Finally, we examined cellular expression profiles of OBP4 in putative antennal sensilla that are related to female A. lineolatus host plant location. The fluorescence in situ hybridization and immunocytochemical labeling assay showed that the OBP4 gene was highly expressed in the multiporous olfactory sensilla medium-long sensilla basiconica rather than in the short sensilla basiconica or uniporous sensilla chaetica. These results, together with those of our previous studies, indicate that OBP4 not only functions in recognition of bug-produced sex pheromones in males, but is probably involved in detection of host plant volatiles in both A. lineolatus sexes. Our findings support the hypothesis that the origin and function of PBPs in hemipteran bugs differ from those of well-known PBPs in lepidopteran moths, which provides a novel perspective on evolutionary mechanisms of sex pheromone communication across insect orders.