Development of a convenient immunochromatographic strip for the diagnosis of infection with Japanese encephalitis virus in swine
Development of a convenient immunochromatographic strip for the diagnosis of infection with Japanese encephalitis virus in swine
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DOI：
10.1016/j.jviromet.2010.04.015
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发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Cao, Shengbo
中科院分区：
文献类型：
--
作者：
Li, Yaoming;Hou, Lidan;Cao, Shengbo
日本脑炎（JE）是由日本脑炎病毒（JEV）引起的。这是亚洲的一个重大公共卫生问题。乙脑病毒感染猪可引起母猪致死性脑炎、妊娠母猪流产、死胎和公猪精子减少。猪是病毒的放大器，因此在JEV传播中起着关键作用。因此，需要开发一种快速检测猪JEV的方法来进行临床JE诊断并抑制病毒传播。本研究利用抗乙脑病毒E蛋白的单克隆抗体（2A2和4D1）研制了一种简便、快速的检测乙脑病毒的免疫层析试纸条（ICS）。结果表明，胶体金标记的单抗2A2（CG-MAb）与JEV结合，形成的复合物被另一单抗4D1固定在检测线上，产生红紫色条带。敏感性试验表明，ICS可以检测2.5 × 10（5）PFU的JEV。临床筛选结果表明，ICS的特异性和敏感性分别为99.3%和85.7%。这表明，单克隆抗体为基础的ICS测试可以作为一种方便的方法，用于快速检测感染的猪样品中的JEV。(C)2010爱思唯尔有限公司版权所有。
Japanese encephalitis (JE) is caused by the Japanese encephalitis virus (JEV). It is a major public health problem in Asia. JEV infects swine which results in fatal encephalitis, abortion and stillbirth in pregnant sow, and hypospermia in boars. Swine is a viral amplifier, and thus plays a critical role in JEV transmission. Thus, development of a rapid method for JEV detection in swine is required for clinical JE diagnosis, as well as to suppress viral spread. In this study, a convenient and rapid immunochromatographic strip (ICS) was developed for detecting JEV in swine using two monoclonal antibodies (MAbs) (2A2 and 4D1) against the E protein of JEV. Results showed that colloidal gold-conjugated MAbs 2A2 (CG-MAb) bond with JEV and the resulting complex was held by the other MAb 4D1 at the test line to give a reddish-purple band. Sensitivity tests demonstrated that ICS can detect 2.5 x 10(5) PFU of JEV. The clinical screening results showed that the specificity and sensitivity of the ICS were 99.3% and 85.7% respectively as compared to that of RT-PCR. This suggests that the MAbs-based ICS test can be used as a convenient method for the rapid detection of JEV in infected swine samples. (C) 2010 Elsevier B.V. All rights reserved.