Identification of novel biomarkers of heavy ion exposure: Proteins, miRNAs and tRNA-derived fragments in serum
Identification of novel biomarkers of heavy ion exposure: Proteins, miRNAs and tRNA-derived fragments in serum
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鉴定重离子暴露的新型生物标志物：血清中的蛋白质、miRNA 和 tRNA 衍生片段
DOI：
10.1016/j.actaastro.2021.05.047
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发表时间：
2021-06-08
影响因子：
3.5
通讯作者：
Wei, Wenjun
中科院分区：
文献类型：
--
作者：
Bai, Hao;Zhang, Tongshan;Wei, Wenjun
空间辐射是载人航天的一个主要问题。特别是宇宙射线中的重离子，与X射线、γ射线等常规辐射相比，可导致更严重的DNA损伤，其致癌作用也不确定。能够在早期阶段容易且快速地检测的微创生物标志物对于航天员空间辐射暴露的风险评估是有价值的。来源于组织和血细胞的血清分子已成为理想的生物标志物。然而，对低剂量重离子辐射有响应的分子还未见报道。在这里，小鼠全身暴露于不同剂量的碳离子，线性能量转移（LET）为30 keV/μ m。采用小鼠抗体芯片和RNA测序技术检测照射后24 h血清蛋白和小分子非编码RNA（sncRNA）的表达谱。经条件筛选和进一步验证，2种蛋白、3种miRNA和2种tRNA衍生片段的表达随剂量的增加而明显增加或减少，即使在低至0.1戈伊或0.5戈伊的碳离子辐射小鼠中也是如此。在这项研究中，我们发现了一些新的蛋白质，miRNA和tRNA衍生片段在血清中显示出很大的潜力，作为早期的生物标志物暴露于高能重离子。这些结果对深空探测中重离子辐照的剂量重建和风险评估有一定的参考价值。
Space radiation is a major concern for human spaceflight. Especially, the heavy ions in cosmic rays can result in more serious DNA damage and uncertain consequences in carcinogenesis comparing with conventional radiation such as X- or gamma-rays. Minimally invasive biomarkers that can be easily and quickly detected at an early stage are valuable for risk assessment of astronauts' space radiation exposure. Serum molecules originating from tissues and blood cells have been emerging as ideal biomarkers. However, the molecules that respond to low dose of heavy ion radiation have not been reported. Here, mice were total-body exposed to different doses of carbon ions with linear energy transfer (LET) of 30 keV/mu m. Mouse Antibody Array and RNA sequencing were performed to detect expression profiles of serum proteins and small non-coding RNAs (sncRNAs) at 24 h post-irradiation. After conditional screening and further validation, the expression of 2 proteins, 3 miRNAs and 2 tRNA-derived fragments showed obvious increase or decrease with increasing dose, even in the mice that received as low as 0.1 Gy or 0.5 Gy of carbon ion radiation. In this study, we identified some new proteins, miRNAs and tRNA-derived fragments in serum showing great potential as early biomarkers of exposure to energetic heavy ions. These results might be useful to dose reconstruction and risk assessment of heavy ion exposure in deep space exploration.