Pyrosequencing of Plaque Microflora In Twin Children with Discordant Caries Phenotypes.
Pyrosequencing of Plaque Microflora In Twin Children with Discordant Caries Phenotypes.
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龋齿表型不一致的双胞胎儿童菌斑微生物区系的焦磷酸测序
DOI：
10.1371/journal.pone.0141310
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Du M
中科院分区：
文献类型：
--
作者：
Zhang M;Chen Y;Xie L;Li Y;Jiang H;Du M
尽管最近在控制龋齿方面取得了成功，但龋齿发展的机制仍不清楚。为了探讨龋齿的原因，特别是在儿童早期龋齿，龈上菌群组成的20个双胞胎不一致的龋齿表型进行了分析，使用高通量焦磷酸测序。此外，父母还完成了一份生活方式问卷。共228，789个测序读数显示10门84属155种微生物菌群，这些菌株的相对丰度在儿童中变化很大，组间比较分析表明，韦荣氏球菌属，棒状杆菌属和放线菌属被认为是龋齿相关的属，梭杆菌属，金氏杆菌属和细毛菌属被认为是健康相关的属，而这6个属之间差异无统计学意义（P>0.05）。此外，聚类分析显示，同一组样本的微生物组成通常不同，但在双胞胎中观察到的微生物组成通常相似。虽然强烈影响龋齿的微生物组成的遗传和环境因素仍然未知，我们推测遗传因素主要影响个体对龋齿的易感性，环境因素主要调节牙菌斑的微生物组成和龋齿的进展。通过使用改进的双胞胎模型和增加样本量，我们的研究可以扩展到分析影响龋齿发展的特定遗传和环境因素。
Despite recent successes in the control of dental caries, the mechanism of caries development remains unclear. To investigate the causes of dental decay, especially in early childhood caries, the supragingival microflora composition of 20 twins with discordant caries phenotypes were analyzed using high-throughput pyrosequencing. In addition, the parents completed a lifestyle questionnaire. A total of 228,789 sequencing reads revealed 10 phyla, 84 genera, and 155 species of microflora, the relative abundances of these strains varied dramatically among the children, Comparative analysis between groups revealed that Veillonella, Corynebacterium and Actinomyces were presumed to be caries-related genera, Fusobacterium, Kingella and Leptotrichia were presumed to be healthy-related genus, yet this six genera were not statistically significant (P>0.05). Moreover, a cluster analysis revealed that the microbial composition of samples in the same group was often dissimilar but that the microbial composition observed in twins was usually similar. Although the genetic and environmental factors that strongly influence the microbial composition of dental caries remains unknown, we speculate that genetic factors primarily influence the individual's susceptibility to dental caries and that environmental factors primarily regulate the microbial composition of the dental plaque and the progression to caries. By using improved twins models and increased sample sizes, our study can be extended to analyze the specific genetic and environmental factors that affect the development of caries.